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Decolorization of orange G byPleurotus ostreatus monokaryotic isolates with different laccase activity

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Abstract

The effect of enhanced laccase (Lac) activity (obtained after copper addition to cultivation media) on decolorization of azo dye Orange G in two basidiospore-derived monokaryotic isolates ofPleurotus ostreatus was determined. The high Lac-producing isolate efficiently decolorized Orange G. The low-producing isolate showed only poor decolorization ability during cultivation in liquid medium and no decolorization on agar plates containing Orange G after a 25-d growth. A substantial enhancement of Lac activity caused by copper addition into cultivation media was detected in both isolates but, at the same time, the biomass production decreased and decolorization rate was reduced.

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Authors and Affiliations

  1. Institute of Microbiology, Academy of Sciences of the Czech Republic, 142 20, Prague, Czechia

    I. Eichlerová, L. Homolka & F. Nerud

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  1. I. Eichlerová
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  2. L. Homolka
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  3. F. Nerud
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Correspondence to I. Eichlerová.

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This work was supported by grant no. 206/02/D119 from theGrant Agency of the Czech Republic and byInstitutional Research Concept no. AV 0Z 502 0903.

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Eichlerová, I., Homolka, L. & Nerud, F. Decolorization of orange G byPleurotus ostreatus monokaryotic isolates with different laccase activity. Folia Microbiol 48, 775–779 (2003). https://doi.org/10.1007/BF02931513

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  • DOI: https://doi.org/10.1007/BF02931513

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