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Production of manganese-dependent peroxidase in a new solid-state bioreactor byPhanerochœte chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes

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Abstract

The production of manganese-dependent peroxidase (MnP) byPhanerochœte chrysosporium in a new solid-state bioreactor, the immersion bioreactor, operating with lignocellulosic waste, such as wood shavings, was investigated. Maximum MnP and lignin peroxidase (LiP) activity of 13.4 and 8.48 μkat/L were obtained, respectively. Thein vitro decolorization of several synthetic dyes by the extracellular liquid produced in the above-mentioned bioreactor (containing mainly MnP) was carried out and its degrading ability was assessed. The highest decolorization was reached with Indigo Carmine (98%) followed by Bromophenol Blue (56%) and Methyl Orange (36%), whereas Gentian Violet was hardly decolorized (6%).

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Abbreviations

MnP:

manganese-dependent peroxidase (EC 1.11.1.13)

LiP:

lignin peroxidase (EC 1.11.1.14)

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Authors and Affiliations

  1. Department of Chemical Engineering, University of Vigo, Lagoas Marcosende, 36200, Vigo, Spain

    S. Rodríguez Couto, A. Domínguez & Á. Sanromán

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  1. S. Rodríguez Couto
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  2. A. Domínguez
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  3. Á. Sanromán
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Rodríguez Couto, S., Domínguez, A. & Sanromán, Á. Production of manganese-dependent peroxidase in a new solid-state bioreactor byPhanerochœte chrysosporium grown on wood shavings. Application to the decolorization of synthetic dyes. Folia Microbiol 47, 417–421 (2002). https://doi.org/10.1007/BF02818701

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