Abstract
The results are reported for the first time on successful plant regeneration from mesophyll-derived protoplasts of ‘rapid cycling’ B. oleracea. Comparative data were also presented on plant regeneration from mesophyll-derived protoplasts of two other varieties namely var. botrytis and var. gemmifera. It was found that a modified Pelletier (Pelletier et al. 1983) protocol is highly beneficial for protoplast culture and plant regeneration from mesophyll-derived protoplasts. The plating efficiency of B. oleracea ‘rapid cycling’ protoplasts was, in the best combination of isolation method, culture technique and culture media 4.5%±0.4% and the plant regeneration frequency approximately 15%. Plant regeneration was further improved by transferring the calli from the D medium of Pelletier to a callus growth medium (MS11) and subsequently to the K3 regeneration medium of Glimelius (Glimelius 1984). Various factors influencing plating efficiency and plant regeneration from mesophyll protoplasts of B. oleracea are discussed.
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Abbreviations
- BAP:
-
6-benzylaminopurine
- IAA:
-
3-indoleacetic acid
- IBA:
-
3-indole butyric acid
- NAA:
-
1-naphtylacetic acid
- 2iP:
-
N6-(2-isopentyl)adenine
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Kik, C., Zaal, M.A.C.M. Protoplast culture and regeneration from Brassica oleracea ‘rapid cycling’ and other varieties. Plant Cell Tiss Organ Cult 35, 107–114 (1993). https://doi.org/10.1007/BF00032959
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DOI: https://doi.org/10.1007/BF00032959