Summary
Genetically transformed cabbage (Brassica oleracea var. capitata) roots were obtained after inoculation with two engineered Agrobacterium rhizogenes strains, each harbouring a plant selectable marker gene in their T-DNA. Axenic root clones resistant to kanamycin or hygromycin B were established, most of which did not exhibit the phenotypic characteristics of Ri-transformed roots. Shoot regeneration was induced from roots after treatment with 2,4-dichlorophenoxyacetic acid (2,4-D). The resulting plants exhibited various phenotypes: some looked normal, while others showed the transformed phenotype observed in other species. Direct evidence for genetic transformation was obtained by molecular hybridization. The trait was transmitted to the progeny. Transformed cabbage plants can be obtained within 6 months using this approach.
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References
Ackermann C (1977) Plant Sci Lett 8: 23–30
Alt-Moerbe J, Neddermann P, von Lintig J, Weiler EW, Schröder J (1988) Mol Gen Genet 213: 1–8
Berthomieu P, Meyer C (1991) Plant Mol Biol 17: 555–557
Brasileiro ACM, Leplé J-C, Muzzin J, Ounnoughi D, Michel M-F, Jouanin L (1991) Plant Mol Biol 17: 441–452
David C, Tempé J (1988) Plant Cell Reports 7: 88–91
De Block M, De Brouwer D, Tenning P (1989) Plant Physiol 91: 694–701
De Framond AJ, Back EW, Chilton WS, Kayes L, Chilton M-D (1986) Mol Gen Genet 202: 125–131
Dellaporta SL, Woods J, Hicks JB (1983) Plant Mol Biol Rep 1: 19–21
Edwards K, Johnstone C, Thompson C (1991) Nucleic Acids Res 19: 1349
Ellis JG, Murphy PH (1981) Mol Gen Genet 181: 36–43
Gritz L, Davies J (1983) Gene 25: 179–180
Guerche P, Jouanin L, Tepfer D, Pelletier G (1987) Mol Gen Genet 206: 382–386
Hosoki T, Shiraishi K, Kigo T, Ando M (1989) Scientia Hortic 40: 259–266
Huffman GA, White FF, Gordon MP, Nester EW (1984) J Bacteriol 157: 269–276
Jouanin L (1984) Plasmid 12: 91–102
Mazodier P, Cossart P, Giraud E, Gasser F (1985) Nucleic Acids Res 13: 195–205
McDonnell RE, Clark RD, Smith WA, Hinchee MA (1987) Plant Mol Biol Rep 5: 380–386
Morel G, Wetmore RH (1951) Amer J Bot 38: 141–143
Murashige T, Skoog F (1962) Physiol Plant 15: 473–497
Petit A, David C, Dahl GA, Ellis JG, Guyon P, Casse-Delbart F, Tempé J (1983) Mol Gen Genet 190: 204–214
Ream W (1989) Annu Rev Phytopathol 27: 583–618
Robaglia C, Vilaine F, Pautot V, Raimond F, Amselem J, Jouanin L, Casse-Delbart F, Tepfer M (1987) Biochimie 69: 231–237
Schmülling T, Schell J, Spena A (1988) EMBO J 7: 2621–2629
Shahin EA, Sukhapinda K, Simpson RB, Spivey R (1986) Theor Appl Genet 72: 770–777
Slightom JL, Durand-Tardif M, Jouanin L, Tepfer D (1986) J Biol Chem 261: 108–121
Srivastava V, Reddy AS, Guha-Mukherjee S (1988) Plant Cell Reports 7: 504–507
Tepfer D (1984) Cell 37: 959–967
Tepfer M, Casse-Delbart F (1987) Microbiological Sciences 4: 24–28
Toriyama K, Stein JC, Nasrallah ME, Nasrallah JB (1991) Theor Appl Genet 81: 769–776
Trail F, Richards C, Wu F-S (1989) In: Bajaj YPS (ed) Plant Protoplasts and Genetic Engineering II, Biotechnology in Agriculture and Forestry, vol 9, Springer-Verlag Berlin Heidelberg
Williams PH, Hill CB (1986) Science 232: 1385–1389
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Berthomieu, P., Jouanin, L. Transformation of rapid cycling cabbage (Brassica oleracea var. capitata) with Agrobacterium rhizogenes. Plant Cell Reports 11, 334–338 (1992). https://doi.org/10.1007/BF00233360
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DOI: https://doi.org/10.1007/BF00233360