Abstract
With the development of scientific exploration in deep space, human activities will become more frequent, and activity time will be longer in the deep space. The environment alteration may cause severe bone changes of human in deep space. The changes of bone mass caused by spatial microgravity are related to the decrease of osteoblast formation and development in bone tissue, and the decrease of osteoblast formation is related to the down-regulation of differentiation of human bone marrow mesenchymal stem cells (hMSCs). Therefore, the study for the biological effects of microgravity on bone cell formation and the relative molecular mechanisms at stem cell level is one of the important subjects to explore the effects of spatial microgravity on bone changes. These studies may provide a scientific basis for the development and the related technologies of target drugs research. Based on exploring the flight conditions on the ground and simulating flight experiments with the automated space experimental device, we utilized a real microgravity environment in the SJ-10 recoverable microgravity experimental satellite (SJ-10 satellite) to examine the effects of space microgravity on transcriptome expression and differentiation potentials of hMSCs.
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Abbreviations
- ALP:
-
Alkaline phosphatase
- BMP2:
-
Bone morphogenetic protein-2
- ECM:
-
Extracellular matrix
- FBS:
-
Heat-inactivated fetal bovine serum
- GADD45:
-
Growth arrest and DNA damage inducible alpha
- GO:
-
Gene ontology
- hBMSCs:
-
Human bone marrow mesenchymal stem cells
- LSM:
-
Lymphocyte separation medium
- MMP1:
-
Matrix metallopeptidase 1
- MSCs:
-
Mesenchymal stem cells
- NG:
-
Normal ground gravity
- PBS:
-
Phosphate buffer saline
- PLGA:
-
Synthetic poly (D, L-lactide-co-glycolide)
- qRT-PCR:
-
Quantitative real-time PCR
- RNA-SEQ:
-
RNA sequence
- RPM:
-
Random positioning machine
- SEM:
-
Scanning electronic microscope
- SMG:
-
Simulated microgravity
- α-MEM:
-
Α-minimum essential medium
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Acknowledgements
We much thank the opportunity of space experiment supplied by SJ-10 Recoverable Scientific Satellite. This study was supported by the grants from Strategically Guiding Scientific Special Project from Chinese Academy of Sciences (XDA04020202-23), Chinese National Nature Science Foundation (U1738102, 81570932).
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Zhang, C., Li, L., Wang, J. (2019). Effects of Space Microgravity on the Trans-differentiation Between Osteogenesis and Adipogenesis of Human Marrow-Derived Mesenchymal Stem Cells. In: Duan, E., Long, M. (eds) Life Science in Space: Experiments on Board the SJ-10 Recoverable Satellite. Research for Development. Springer, Singapore. https://doi.org/10.1007/978-981-13-6325-2_12
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