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Intramuscular injection of mechano growth factor E domain peptide regulated expression of memory-related sod, miR-134 and miR-125b-3p in rat hippocampus under simulated weightlessness

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Abstract

Objective

To investigate the expression of memory-related antioxidant genes and miRNAs under simulated weightlessness and the regulation of mechano growth factor (MGF) E domain, the peptide preventing nerve damage.

Results

Igf-iea and mgf mRNA levels, expression of antioxidant genes sod1 and sod2 and levels of miR-134 and miR-125b-3p increased in rat hippocampus after 14 days tail suspension to simulate weightlessness which was inhibited with intramuscular injection of E domain peptide. Therefore, administration of MGF E domain peptide could reverse increased expressions of memory-related igf-iea, mgf, sod1, sod2, miR-134 and miR-125b-3p in rat hippocampus under simulated weightlessness.

Conclusions

MGF may regulate the redox state and miRNA-targeted NR-CREB signaling, and intramuscular injection may be the alternative administration because of its safety, convenience and ability to pass through the blood brain barrier.

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Acknowledgments

This work was supported by the National instrumentation program of China (2013YQ190467, 2012YQ040140091), Shenzhen science & technology institute (JCYJ20150629164441050), Foundation of State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center (SMFA15B01, SMFA13B02) and the Medicinal Science and Technology Research Project (BWS11J052).

Supporting information

Supplementary Table 1—sequences of qPCR primers for rats

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Authors and Affiliations

  1. State Key Laboratory of Space Medicine Fundamentals and Application, China Astronaut Research and Training Center, 5132-23#, Beiqing Road 26#, Haidian District, Beijing, 100094, China

    Hailong Chen, Ke Lv, Zhongquan Dai, Guohua Ji, Tingmei Wang, Yanli Wang, Yongliang Zhang, Guanghan Kan, Yinghui Li & Lina Qu

  2. Department of Health Technology Research and Development, Space Institute of Southern China (Shenzhen), Shamiao Road 4#, Pingdi Street, Longgang District, Shenzhen, 518117, China

    Hailong Chen

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  1. Hailong Chen
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Correspondence to Yinghui Li or Lina Qu.

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Chen, H., Lv, K., Dai, Z. et al. Intramuscular injection of mechano growth factor E domain peptide regulated expression of memory-related sod, miR-134 and miR-125b-3p in rat hippocampus under simulated weightlessness. Biotechnol Lett 38, 2071–2080 (2016). https://doi.org/10.1007/s10529-016-2210-4

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