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Effects of Microgravity Modeled by Large Gradient High Magnetic Field on the Osteogenic Initiation of Human Mesenchymal Stem Cells

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Abstract

Microgravity (MG) leads to a decrease in osteogenic potential of human bone marrow-derived mesenchymal stem cells (hMSCs). In the present study, we used large gradient high magnetic field (LGHMF) produced by a superconducting magnet to model MG (LGHMF-MG) and analyzed the effects of LGHMF-MG on survival, cytoskeleton and osteogenic potential of hMSCs. Results showed that the LGHMF-MG treatment for 6 h disrupted the cytoskeleton of hMSCs, and the LGHMF-MG treatment for 24 h led to cell death. LGHMF-MG treatments for 6 h in early stages of osteogenic induction (the pre-treatment before osteogenic induction, the beginning-treatment in the beginning-stage of osteogenic induction and the middle-treatment in the middle-stage of osteogenic induction) resulted in suppression on osteogenesis of hMSCs. The suppression intensity was reduced gradually as the treatment stage of LGHMF-MG was postponed. The LGHMF-MG treatment for 6 h in the ending-stage of osteogenic induction (the ending-treatment) had no obvious effect on osteogenesis of hMSCs. These results indicated that LGHMF-MG should affect the initiation of osteogenesis. Finally, the possible mechanism for the inhibition effect of LGHMF-MG on osteogenesis of hMSCs is discussed.

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Acknowledgments

We would like to thank Dr. Chris Wood in Zhejiang University and Dr. Hongqi Liu in Thomas Jefferson University for critical reading of the manuscript. This work was supported by Scientific Research from the Scientific Foundation of Zhejiang (2009C13020), National Science Fund of China (30971460).

Disclosures

The authors indicate no potential conflicts of interest.

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Authors and Affiliations

  1. Institute of Cell Biology and Genetics, College of Life Sciences, Zhejiang University, Room 307, Building of Life Sciences, Zijingang Campus, No.388, Yuhangtang Road, Hangzhou, Zhejiang, 310058, People’s Republic of China

    Dongyan Shi, Wanglong Deng, Wenji Yuan, Liyue Liu, Chen Zong & Jinfu Wang

  2. Key Laboratory for Space Biosciences & Biotechnology, Faculty of Life Sciences, Northwestern Polytechnical University, 127 Youyi Xilu, Xian, Shangxi, 710072, People’s Republic of China

    Rui Meng & Peng Shang

  3. Systems Biology Division, Zhejiang-California International Nanosystems Institute (ZCNI) of Zhejiang University, Huajiachi Campus, Hangzhou, Zhejiang, 310029, People’s Republic of China

    Wenchao Ding

  4. The Second Hospital, Zhejiang University, Hangzhou, Zhejiang, 310009, People’s Republic of China

    Qiang Zheng

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  1. Dongyan Shi
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Correspondence to Peng Shang or Jinfu Wang.

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Shi, D., Meng, R., Deng, W. et al. Effects of Microgravity Modeled by Large Gradient High Magnetic Field on the Osteogenic Initiation of Human Mesenchymal Stem Cells. Stem Cell Rev and Rep 6, 567–578 (2010). https://doi.org/10.1007/s12015-010-9182-x

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