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Plant Trait Gene Expression Cassette Design

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Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants

Abstract

Plant biotechnology depends on robust control of trait gene expression to produce an adequate level of protein or RNA in the appropriate cells at the correct time. Early biotechnology products depended on high protein production in most plant cells, and relied on relatively few well-characterized “promoters.” This demand continues and has expanded into other classes of expression control, such as tissue specificity and environmental responsiveness, as new trait technologies are pursued. Genomic information can be leveraged to meet this demand now and well into the future. A method has been developed of identifying donor genes in genomic data, and simplifying their annotation to facilitate design and development of effective expression cassettes. This approach greatly reduces the effort and expense of producing novel trait gene expression tools. Coupled with chemical DNA synthesis technology, this approach makes it possible to develop reliable expression cassettes from virtually any plant gene.

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Authors and Affiliations

  1. Syngenta Biotechnology, Inc., 3054 East Cornwallis Road, Research Triangle Park, 27709, NC, Research Triangle Park, USA

    Michael Nuccio, Jared Conville, Ailing Zhou & Xiaomei Liu

  2. Syngenta Biotechnology (China) Co. Ltd, Zhongguancun Life Science Park, No. 25, Life Science Park Road, Changping District, 102206, Beijing, China

    Xi Chen

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  1. Michael Nuccio
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  3. Jared Conville
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  4. Ailing Zhou
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Correspondence to Michael Nuccio .

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  1. Syngenta Biotechnology Inc., Research Triangle Park, North Carolina, USA

    Kasi Azhakanandam

  2. Syngenta Biotechnology, Inc., Research Triangle Park, North Carolina, USA

    Aron Silverstone

  3. Departments of Biochemistry and Pathology, University of Pennsylvania, Philadelphia, Pennsylvania, USA

    Henry Daniell

  4. Plant and Crop Sciences Division, University of Nottingham School of Biosciences, Loughborough, United Kingdom

    Michael R. Davey

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Nuccio, M., Chen, X., Conville, J., Zhou, A., Liu, X. (2015). Plant Trait Gene Expression Cassette Design. In: Azhakanandam, K., Silverstone, A., Daniell, H., Davey, M. (eds) Recent Advancements in Gene Expression and Enabling Technologies in Crop Plants. Springer, New York, NY. https://doi.org/10.1007/978-1-4939-2202-4_2

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