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Population-level analysis of leaf anatomy of Daustinia montana (Moric.) Buril & A.R. Simões: a polymorphic species with tangled taxonomic history

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Abstract

Daustinia montana (Moric.) Buril & A.R. Simões (Convolvulaceae) is currently classified as a highly polymorphic species with remarkable variations of leaf morphology, in terms of their sizes, shapes, margins, and indument. This foliar variability has led taxonomists to classify this taxon either as more than one species, with many varieties, or—more recently—as a single broadly polymorphic species. Previous morphometric analyses had correlated those variations to population distributions, with leaves becoming wider further south through its distribution range. We herein investigated whether leaf anatomy follows the wide variability of external morphology, or if it retains more preserved characters by performing an analysis at the populational level. This is the most complete morphoanatomical description of the species. We analyzed specimens from 16 populations, representing the full extent of the occurrence of D. montana, using light and scanning electron microscopy, and performed a cluster analysis with six bioclimatic variables. Several characters were found as variable among the population, such as smaller bundles, stomata types, as well as the presence of endodermal structures surrounding the vascular system in the mesophyll region. The population from Maraú, in Bahia State, is the most disparate one by being the only one that presents cyclocytic stomata, straight-to-curve epidermal cell, and collateral accessory bundles in the petioles. The results found suggest correlated with environmental adaptations.

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References

  • Pisuttimarn P, Traiperm P, Pornpongrungrueng P (2013) Comparative anatomy of Merremia section Xanthips (Convolvulaceae) in Thailand (in Thai). In: Proceedings in the national graduate research conference 29th (NGRC29). Chiang Rai, Thailand, pp 93–101

  • Ab’Sáber AN (2003) Os domínios de natureza no Brasil: Potencialidades paisagísticas. Ateliê Editorial, São Paulo

    Google Scholar 

  • Alencar J, Nascimento ALB, Duarte-Neto P, Buril MT (2020) Disentangling leaf morphology in Daustinia montana (Convolvulaceae). Syst Bot 45:921–930

    Article  Google Scholar 

  • Araújo JS, Azevedo AA, Silva LC, Meira RMSA (2010) Leaf anatomy as an additional taxonomy tool for 16 species of Malpighiaceae found in the Cerrado area (Brazil). Plant Syst Evol 286:117–131

    Article  Google Scholar 

  • Austin DF (1978) The Ipomoea batatas complex-I. taxonomy. Bull Torrey Bot Club 105(2):114–129

    Article  Google Scholar 

  • Bhati P, Sem DN (1978) Adaptative polymorphism in Ipomoea pes-tigridis (Convolvulaceae), a common rainy season weed of the Indian arid zone. Plant Syst Evol 129:111–117

    Article  Google Scholar 

  • Bolarinwa KA, Oyebanji OO, Olowokudejo JD (2018) Comparative morphology of leaf epidermis in the genus Ipomoea (Convolvulaceae) in Southern Nigeria. Ann West Univ Timiş, Ser Biol 21:29–46

    Google Scholar 

  • Bukatsch F (1972) Bemerkungen zur doppelfarbung. Microkosmos 61:255

    Google Scholar 

  • Buril MT, Alves M (2013) Jacquemontia diamantinensis sp. nov. (Convolvulaceae) from the Chapada Diamantina. Brazil Nord J Bot 30:001–004

    Google Scholar 

  • Buril MT, Simões AR, Carine M, Alves M (2014) Austinia, a new genus of Convolvulaceae from Brazil. Phytotaxa 186:254–260

    Article  Google Scholar 

  • Buril MT, Simões AR, Carine M, Alves M (2015) Daustinia, a replacement name for Austinia (Convolvulaceae). Phytotaxa 197:060–060

    Article  Google Scholar 

  • Buril MT (2013) Sistemática e filogenia de Jacquemontia (Choisy) Convolvulaceae. PhD Thesis, Universidade Federal de Pernambuco, Recife

  • Campitelli BE, Stinchcombe JR (2013) Natural selection maintains a single-locus leaf shape cline in Ivyleaf morning glory, Ipomoea hederacea. Mol Ecol 22:552–564

    Article  CAS  PubMed  Google Scholar 

  • Castro MM, Machado SR (2003) Células e tecidos secretores. In: Appezzato-da-Glória B, Carmello-Guerreiro SM (eds) Anatomia vegetal. Universidade Federal de Viçosa, Viçosa, pp 179–204

    Google Scholar 

  • Chitchak N, Traiperm P, Staples G, Rattanakrajang P, Sumanon P (2018) Species delimitation of some Argyreia (Convolvulaceae) using phenetic analyses: insights from leaf anatomical data reveal a new species. Botany 96:217–233

    Article  Google Scholar 

  • Choisy JD (1837) Convolvulaceae. Mémoires de la Société de Physique et d'Histoire Naturelle de Genève 8:135–144

    Google Scholar 

  • Dickison WC (2000) Integrative plant anatomy. Academic Press, San Diego

    Google Scholar 

  • Eiten G (1972) The cerrado vegetation of Brazil. Bot Rev 38:201–341

    Article  Google Scholar 

  • Ekeke C, Nichodemus CO, Ogazie CA (2021) Morphological and anatomical studies on Ipomoea coccinea L. (Convolvulaceae): a new record from Nigeria. Asian J Res Bot 6:1–8

    Google Scholar 

  • Esau K (1965) Plant anatomy. John Wiley and Sons, New York

    Google Scholar 

  • Esau K (1974) Anatomia das plantas com sementes. Blücher, São Paulo

    Google Scholar 

  • Farias V, Maranho LT, Mushner VC, Soffiatti P (2016) Anatomia foliar de Passiflora subgênero Decaloba (Passifloraceae): implicações taxonômicas. Rodriguésia 67:029–043

    Article  Google Scholar 

  • Fernández-Mazuecos M, Glover BJ (2017) The evo-devo of plant speciation. Nat Ecol Evol 1:110

    Article  PubMed  Google Scholar 

  • Fick SE, Hijmans RJ (2017) Worldclim 2: New 1-km spatial resolution climate surfaces for global land areas. Int J Climatol 37:4302–4315

    Article  Google Scholar 

  • Fineran BA, Condom JM, Ingerfeld M (1988) An impregnated suberized wall layer in laticifers of the and its resemblance to that in walls of oil cells. Protoplasma 147:42–54

    Article  Google Scholar 

  • Fiser C, Robinson CT, Malard F (2017) Cryptic species as a window into the paradigm shift of the species concept. Mol Ecol 27:613–635

    Article  Google Scholar 

  • Folorunso AE (2013) Taxonomic evaluation of fifteen species of Ipomoea L. (Convolvulaceae) from South-Western Nigeria using foliar micromorphological characters. Not Sci Biol 5:156–162

    Article  Google Scholar 

  • Font Quer P (1953) Diccionario de botánica. Editorial Labor, Barcelona

    Google Scholar 

  • Franceschi VR, Horner HT (1980) Calcium oxalate crystals in plants. Bot Rev 46:361–427

    Article  CAS  Google Scholar 

  • Franceschi VR, Nakata PA (2005) Calcium oxalate in plants: formation and function. Annu Rev Plant Biol 56:41–71

    Article  CAS  PubMed  Google Scholar 

  • Franklin GL (1945) Preparation of thin sections of synthetic resins and wood-resin composites, and a new macerating method for wood. Nature 155:51

    Article  Google Scholar 

  • Gogosz AM, Boeger MRT, Negrelle RRB, Bergo C (2012) Anatomia foliar comparativa de nove espécies do gênero Piper (Piperaceae). Rodriguésia 63:405–417

    Article  Google Scholar 

  • Gomes SMA, Silva EAM, Lombardi JA, Azevedo AA, Vale FHA (2005) Anatomia foliar como subsídio à taxonomia de Hippocrateoideae (Celastraceae) no Sudeste do Brasil. Acta Bot Bras 19:945–961

    Article  Google Scholar 

  • Hallier H (1893) Versuch einer natürlichen gleiderung der Convolvulaceen auf morphologischer und anatomischer grundlage. Bot Jahrb Syst 16:453–591

    Google Scholar 

  • Hsu T-W, Wang K-H, Chiang T-Y (2006) Ipomoea purpurea (L.) Roth (Convolvulaceae), a species of the I. nil complex, newly naturalized in Taiwan. BioFormosa 41:19–22

    Google Scholar 

  • Johansen DA (1940) Plant microtechnique. Mc Graw Hill Book Co. ilnc, New York

    Google Scholar 

  • Ketjarun K, Staples GW, Swangpol SC, Traiperm P (2016) Micro-morphological study of Evolvulus spp. (Convolvulaceae): the old world medicinal plants. Bot Stud 57:25

    Article  PubMed  PubMed Central  Google Scholar 

  • Konno K (2011) Plant latex and other exudates as plant defense systems: roles of various defense chemicals and proteins contained therein. Phytochemistry 72:1510–1530

    Article  CAS  PubMed  Google Scholar 

  • Kuster VC, Silva LC, Meira RMSA, Azevedo AA (2016) Glandular trichomes and laticifers in leaves of Ipomoea pes-caprae and I. imperati (Convolvulaceae) from coastal Restinga formation: Structure and histochemistry. Braz J Bot 39:1117–1125

    Article  Google Scholar 

  • Lewinsohn TM (1991) The geographical distribution of plant latex. Chemoecology 2:64–68

    Article  Google Scholar 

  • Liphschitz N, Waisel Y (1982) Adaptations of plants to saline environments: salt excretion and glandular structure. In: Sen DN, Rajpurohit KS (eds) Tasks for vegetation science. Junk Publishers, pp 197–214

    Google Scholar 

  • Lourenço JAAM, Correia BEF, Diniz MR, Rêgo MMC, Almeida-Jr EB, Buril MT (2020) Ampliação da distribuição de Daustinia montana (Moric.) Buril & A.R. Simões (Convolvulaceae): Registro da primeira ocorrência para o Maranhão Brasil. Biota Amazôn 10:53–55

    Google Scholar 

  • Marques CA, Azevedo AA (2011) Caracterização anatômica da folha de Aniba firmula (Nees & C. Mart.) Mez (Lauraceae). Rev De Cienc Farm Basica e Apl 32:251–256

    Google Scholar 

  • Martins FM, Lima JF, Mascarenhas AAS, Macedo TP (2012) Secretory structures of Ipomoea asarifolia: anatomy and histochemistry. Braz J Pharmacog 22:13–20

    Article  CAS  Google Scholar 

  • Meissner CF (1869) Convolvulaceae: Jacquemontia. In: Martius CPF, Eichler AG (eds), Fl Bras vol 7, pp 304–305

  • Metcalfe CR (1967) Distribution of latex in the plant kingdom. Econ Bot 21:115–127

    Article  Google Scholar 

  • Metcalfe CR, Chalk L (1950) Anatomy of the dicotyledons. Claredon Press, Oxford

    Google Scholar 

  • Milan P, Hayashi AH, Appezzato-da-Glória B (2006) Comparative leaf morphology and anatomy of three Asteraceae species. Braz Arch Biol Technol 49:135–144

    Article  Google Scholar 

  • Mori AS, Silva LAM, Lisboa G, Coradin L (1989) Manual de manejo do herbário fanerogâmico. Centro de Pesquisas do Cacau, Ilhéus

  • Moricand S (1837) Plantes nouvelles d'Amérique. Jules-Gme Fick, Genève 41–56

  • Muhaidat R, Sage RF, Dengler NG (2007) Diversity of Kranz anatomy and biochemistry in C4 Eudicots. Am J Bot 94:362–381

    Article  CAS  PubMed  Google Scholar 

  • Olaranont Y, Stauffer F, Traiperm P, Staples GW (2018) Investigation of the black dots on leaves of Stictocardia species (Convolvulaceae) using anatomical and histochemical analyses. Flora 249:133–142

    Article  Google Scholar 

  • Oliveira MIU, Rebouças DA, Leite KRB, Oliveira RP, Funch LS (2018) Can leaf morphology and anatomy contribute to species delimitation? A case in the Campomanesia xanthocarpa complex (Myrtaceae). Flora 249:111–123

    Article  Google Scholar 

  • Paes LS, Mendonça MS (2008) Aspectos morfo-anatômicos de Bonamia ferrugínea (Choisy) Hallier f. (Convolvulaceae). Rev Bras Pl Med 10:76–82

    Google Scholar 

  • Palhares D, Zaidan LBP (2010) A brief review on leaf anatomy of plants with certain peculiar modes of photosynthesis. Revista Biociênc 16:1

    Google Scholar 

  • Payne WW (1978) A glossary of plant hair terminology. Brittonia 30:239–255

    Article  Google Scholar 

  • Prabhakar M (2004) Structure, delimitation, nomenclature and classification of stomata. Acta Bot Sin 46:242–252

    Google Scholar 

  • Purvis M, Collier D, Walls D (1964) Laboratory techniques in botany. Butterworths, London

    Google Scholar 

  • QGIS Development Team (2015) QGIS Geographic Information System. Open Source Geospatial Foundation Project. Available from: http://www.qgis.org/

  • Roudi E, Khodayari H, Mozaffarian V, Zarre S (2020) Trichome micromorphology and its significance in the systematics of Convolvulus L. (Convolvulaceae). Turk J Botany 44:178–191

    Google Scholar 

  • Sage RF (2001) Environmental and evolutionary preconditions for the origin and diversification of the C4 photosynthetic syndrome. Plant Biol 3:202–213

    Article  CAS  Google Scholar 

  • Sage RF (2004) The evolution of C4 photosynthesis. New Phytol 161:341–370

    Article  CAS  PubMed  Google Scholar 

  • Sage RF, Monson RK (1999) C4 plant biology. Academic Press, San Diego

    Google Scholar 

  • Sampaio EVSB, Giulietti AM, Virgínio J, Gamarra-Rojas CFL (2002) Vegetação e flora da caatinga. Associação Plantas do Nordeste – APNE, Centro Nordestino de Informações sobre Plantas – CNIP, Recife

  • Santos D, Arruda ECP, Buril MT (2020a) Hidden in the rocks: a new species of Evolvulus L. (Convolvulaceae) revealed by anatomy. Brittonia 72:282–289

    Article  Google Scholar 

  • Santos D, Saraiva RVC, Ferraz TM, Arruda ECP, Buril MT (2020b) A threatened new species of Ipomoea (Convolvulaceae) from the Brazilian Cerrado revealed by morpho-anatomical analysis. PhytoKeys 151:93–106

    Article  PubMed  PubMed Central  Google Scholar 

  • Sayeedud-Din M (1953) Observations on the anatomy of some of the Convolvulaceae. Bot J Linn Soc 37:106–109

    Google Scholar 

  • Silva SO, Calaça FJS, Figueiredo ADL (2012) Anatomia foliar de Operculina macrocarpa L. Urban (Convolvulaceae). Heringeriana 6:90–92

    Article  Google Scholar 

  • Simões AR, Culham A, Carine M (2015) Resolving the unresolved tribe: a molecular phylogenetic framework for the Merremieae (Convolvulaceae). Bot J Linn Soc 179:374–387

    Article  Google Scholar 

  • Smith FH, Smith EC (1942) Anatomy of the inferior ovary of Darbya. Am J Bot 29:464–471

    Article  Google Scholar 

  • Solereder H (1908) Systematic anatomy of the dicotyledons I. Claredon Press, Oxford

    Google Scholar 

  • Stefanović S, Austin DF, Olmstead RG (2003) Classification of convolvulaceae: a phylogenetic approach. Syst Bot 28:791–806

    Google Scholar 

  • Thiers B (2021) Continuously updated index herbariorum: a global directory of public herbaria and associated staff. New York Botanical Garden’s virtual herbarium. http://sweetgum.nybg.org/ih/. Accessed 23 March 2021

  • Tooulakou G, Giannopoulos A, Nikolopoulos D, Bresta P, Dotsika E, Orkoula MG, Kontoyannis CG, Fasseas C, Liakopoulos G, Klapa MI, Karabourniotis G (2016) “Alarm photosynthesis”: Calcium oxalate crystals as an internal CO2 source in plants. Plant Physiol 171:2577–2585

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Traiperm P, Chow J, Nopun P, Staples GW, Swangpol SC (2017) Identification among morphologically similar Argyreia (Convolvulaceae) based on leaf anatomy and phenetic analyses. Bot Stud 58:25

    Article  PubMed  PubMed Central  Google Scholar 

  • Weiglin C, Winter E (1988) Studies on the ultrastructure and development of the glandular trichomes of Cressa cretica L. Flora 181:19–27

    Article  Google Scholar 

  • Welkie GW, Caldwell M (1970) Leaf anatomy of species in some dicotyledon families as related to the C3 and C4 pathways of carbon fixation. Can J Bot 48:2135–2146

    Article  Google Scholar 

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Acknowledgements

The authors thanks Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) for granting the first author's scholarship (DS 88882.436289/2019-01); to Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) for funding the project “Convolvulaceae of South America” (PVE 314725/2014-8); to Instituto Chico Mendes de Conservação da Biodiversidade (ICMBIO) for collection authorizations; the managers of the federal or state protected areas visited; to herbaria curators; the “Pesquisa em Movimento” Program at the Universidade Federal Rural de Pernambuco (UFRPE); to the anonymous reviewers for their collaboration in improving the manuscript; to Bruna Emanuele Freire Correia, Maira Rodrigues Diniz, Renara Nichio Amaral and Leonardo Jales and Integrative Systematic Laboratory team (LASI / UFRPE) for their collaboration in field expeditions.

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Authors and Affiliations

  1. Laboratório de Sistemática de Angiospermas, Programa de Pós Graduação Em Botânica, Departamento de Biologia, Universidade Federal Rural de Pernambuco, R. Dom Manuel de Medeiros, s/n, Recife, Pernambuco, Brazil

    Juliana Alencar, Joilson Viana Alves & Maria Teresa Buril

  2. Laboratório de Anatomia Vegetal, Programa de Pós Graduação Em Biologia Vegetal, Departamento de Botânica, Universidade Federal de Pernambuco, Av. Prof. Moraes Rego, 1235 - Cidade Universitária, Recife, Pernambuco, Brazil

    Emília Arruda

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  1. Juliana Alencar
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  4. Maria Teresa Buril
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Contributions

The study conception and design were the responsibility of JA and MTB. JA conducted the research, carried out the botanical collections in the field, made the figures, map, tables, illustrations, and wrote the manuscript. Part of the anatomical analyzes and slides photography were conducted by JVA; MTB supervised the research project; and EA supervised the anatomical analyses and both revised the text. All authors read and approved the final manuscript.

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Correspondence to Juliana Alencar.

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Alencar, J., Alves, J.V., Arruda, E. et al. Population-level analysis of leaf anatomy of Daustinia montana (Moric.) Buril & A.R. Simões: a polymorphic species with tangled taxonomic history. Braz. J. Bot 45, 1279–1295 (2022). https://doi.org/10.1007/s40415-022-00836-x

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