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Rice Straw and Swine Manure Anaerobic co-digestion Enhancement Through Bioaugmentation: Effect on the Microbial Community

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Abstract

The degradation of agricultural residues and their bioconversion to methane is still hampered by the search for pre-treatment strategies due to the lignocellulosic content that limits the process efficiency. Adding an enriched microbial consortium could be an alternative for the biological treatment of lignocellulosic biomass. Therefore, during the degradation process, it is necessary to study the dynamics and structure of the microbial community. The objective of this study was to evaluate the addition of an enriched microbial consortium and its effect on the methane-producing prokaryotic community during the anaerobic co-digestion of rice straw and swine manure. Two 10 L semi-continuous stirred tank reactors were used (control and bioaugmentation), during 70 days at 35 ± 2 °C, increasing organic loading rates up to 1.8 g VS/L/d. The diversity and dynamics of the microbial community were analyzed and taxonomic identification was obtained by Ion Torrent metagenomic technique. A higher production was achieved in the bioaugmented reactor (approx. 344 LN/kgVS) concerning the control reactor, increasing the methane yield by 40% (v/v). The metagenomic method allowed the identification down to the genus and species level of the microbial consortium and the prokaryotic community of the reactors, which enabled the assumption of possible metabolic pathways. In both reactors, species diversity decreased over time, indicating a greater specialization of the microbial community. Cellulolytic and methanogenic acetoclastic microorganisms were favored, which supported the higher methane production obtained. The results demonstrate that the consortium could be used as a bioproduct to treat agricultural wastes for energy purposes.

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Acknowledgements

This research was supported by the Canadian Bureau for International Education (Emerging Leaders in the Americas Program scholarship) with a research grant awarded to Annerys Carabeo-Pérez [grant number 509]. The authors acknowledge the cooperation between the Cuban and Canadian institutions that participated in the results of this research. The authors gratefully acknowledge the support provided by the Research, Development and Innovation Project “Bioaugmentation, biocontrol and biostimulation of two-stage anaerobic digestion to obtain hydrogen and methane”, with code PN211LH005-005. We also extend our thanks to all the individuals and organizations who contributed to this work in various ways.

Funding

This work was supported by the Canadian Bureau for International Education (Emerging Leaders in the Americas Program scholarship) with a research grant awarded to Annerys Carabeo-Pérez [grant number 509].

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Authors and Affiliations

  1. Unidad de Desarrollo e Innovación-Centro de Estudios de Energía y Procesos Industriales, Universidad de Sancti Spíritus “José Martí”, Ave. De los Mártires No. 360, Sancti Spíritus, CP 60 100, Cuba

    Annerys Carabeo-Pérez & Janet Jiménez

  2. Facultad de Biología, Universidad de La Habana, Calle 25 e/ I y J, Vedado CP 10400, La Habana, Cuba

    María Isabel Sánchez López & Gilda Guerra Rivera

  3. Institute for Sustainable Horticulture, Kwantlen Polytechnic University, British Columbia, Surrey, Canada

    Deborah Henderson

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  1. Annerys Carabeo-Pérez
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  2. María Isabel Sánchez López
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  3. Gilda Guerra Rivera
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  4. Deborah Henderson
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  5. Janet Jiménez
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Contributions

Janet Jiménez, María Isabel Sánchez López and Gilda Guerra Rivera contributed to the study conception and design. Deborah Henderson provided the study materials, reagents, laboratory samples, instrumentation, computing resources, and other analysis tools. Material preparation, performing the experiments, data collection and analysis were performed by Annerys Carabeo-Pérez. The first draft of the manuscript was written by Annerys Carabeo-Pérez and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.

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Correspondence to Annerys Carabeo-Pérez.

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Carabeo-Pérez, A., López, M.I.S., Rivera, G.G. et al. Rice Straw and Swine Manure Anaerobic co-digestion Enhancement Through Bioaugmentation: Effect on the Microbial Community. Bioenerg. Res. 17, 756–767 (2024). https://doi.org/10.1007/s12155-023-10676-6

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