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The potential therapeutic effect of klotho on cell viability in human colorectal adenocarcinoma HT-29 cells

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Abstract

Klotho is an anti-aging, anti-inflammator, and anti-oxidative protein and has been shown to important role in tumorigenesis, proliferation, survival, autophagy, and resistance to tumor suppressor effects in several types of human cancers. In this study, we aimed to investigate possible anti-tümör and apoptotic effects of exogen klotho in human colorectal adenocarcinoma cells (HT-29) and healthy colon cells (CCD 841 CoN). The WST-8 test was used to determine the half-maximum inhibitory concentration (IC50) of the klotho protein. AO-PI fluorescent staining techniques and Annexin V-PI flow cytometry was utilized to observe and detect the apoptosis of cancer cells induced by klotho. Our results demonstrated that klotho had a cytotoxic effect against colorectal adenocarcinoma cells in a dose-dependent manner. Our Annexin V-PI flow cytometric and AO-PI fluorescent analyses showed that klotho induced quantitative and morphological changes that indicate apoptotic induction in the human colorectal adenocarcinoma. This study results proved for the first time that klotho may be an effective potential therapeutic agent that may be used in adjuvant therapy in human colorectal adenocarcinoma it does not affect selectively healthy colon cells and but leading cancer cells to apoptosis.

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Acknowledgements

We thank to Mehtap Kutlu and Alper Tunga Ozdemir for the donation of cell lines.

Funding

This study was supported by grants Eskişehir Osmangazi University, Scientific Research Projects (ESOGU-BAP, ESTEM Code: 202046A113).

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Authors and Affiliations

  1. Cellular Therapy and Stem Cell Production Application, Research Centre (ESTEM), Institute of Health Sciences, Eskisehir Osmangazi University, 26040, Eskisehir, Turkey

    Ayla Eker Sariboyaci, Onur Uysal, Merve Nur Soykan & Sibel Gunes

  2. Department of Stem Cell, Institute of Health Sciences, Eskisehir Osmangazi University, Eskisehir, Turkey

    Ayla Eker Sariboyaci, Onur Uysal, Merve Nur Soykan & Sibel Gunes

  3. Vocational School of Health Services, Eskisehir Osmangazi University, Eskisehir, Turkey

    Ayla Eker Sariboyaci, Onur Uysal & Sibel Gunes

Authors
  1. Ayla Eker Sariboyaci
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  2. Onur Uysal
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  3. Merve Nur Soykan
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  4. Sibel Gunes
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Contributions

AES is the main author in this study and contributed to the study conception and design. Material preparation, data collection, and analysis were performed by SG and MNS. The first draft of the manuscript was written by OU. All authors commented on previous versions of the manuscript. Corresponding author read and approved the final manuscript.

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Correspondence to Sibel Gunes.

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The authors report no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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Sariboyaci, A.E., Uysal, O., Soykan, M.N. et al. The potential therapeutic effect of klotho on cell viability in human colorectal adenocarcinoma HT-29 cells. Med Oncol 39, 191 (2022). https://doi.org/10.1007/s12032-022-01793-x

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  • DOI: https://doi.org/10.1007/s12032-022-01793-x

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