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A “Turn-on” Fluorescent Probe Based on Phenothiazine for Selectively Recognizing ClO− and its Practical Applications

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Abstract

Hypochlorous acid (HClO), a highly reactive oxygen species, has important effects on human health. High selectivity and sensitivity remain challenges of fluorescent probes for detection of ClO− with a large Stokes shift. This work designed and synthesized a novel phenothiazine-based fluorescent probe TF which can detect ClO− by colorimetric and fluorescent dual signals. TF displayed turn-on fluorescence effect toward ClO− with high selectivity (≥ 28-folds) and sensitivity (LOD = 0.472 μM), fast response time (< 1 min) and large Stokes shift (150 nm) in PBS (pH = 7.4, 40% DMSO). Meanwhile, TF can visualize ClO− on the mung bean sprouts model and apply as testing strips for portable and rapid detecting ClO− by the naked eyes.

Graphical Abstract

A phenothiazine-based fluorescent probe with large Stokes shift was synthesized and its responding rapidly ability to detect ClO− was studied.

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Funding

The authors are grateful for the financial support from the National Natural Science Foundation of China (41867052, 41867053, 22263005) and the Project of the Science Funds of Jiangxi Education Office (GJJ190613, GJJ211135).

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Author notes

  1. Qianling Liu and Xue Li are contributed equally to this work.

Authors and Affiliations

  1. Jiangxi Key Laboratory of Organic Chemistry, Jiangxi Science and Technology Normal University, Nanchang, 330013, People’s Republic of China

    Qianling Liu, Xue Li, Ming Xiao, Yin Ai, Gang Liu, Haichang Ding & Shouzhi Pu

  2. Department of Ecology and Environment, Yuzhang Normal University, Nanchang, 330103, People’s Republic of China

    Shouzhi Pu

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  1. Qianling Liu
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  2. Xue Li
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  3. Ming Xiao
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Contributions

Qianling Liu. Writing-original draft, Editing. Xue Li. Writing-original draft, Editing. Ming Xiao. Investigation. Yin Ai. Investigation. Gang Liu. Supervision, Review. Haichang Ding. Editing, Review. Shouzhi Pu. Resources, Supervision.

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Correspondence to Haichang Ding or Shouzhi Pu.

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Liu, Q., Li, X., Xiao, M. et al. A “Turn-on” Fluorescent Probe Based on Phenothiazine for Selectively Recognizing ClO− and its Practical Applications. J Fluoresc 33, 2451–2459 (2023). https://doi.org/10.1007/s10895-023-03215-1

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