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Design and Synthesis of Novel Fluorescent Probe Based on Cyanobiphenyl and its Application in Detection of Hypochlorite

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Abstract

Hypochlorite is an important biological reactive oxygen species, which plays a pivotal role in various life activities. Excessive presence in the human body or excessive intake in life causes a series of diseases. To monitor the hypochlorite level in living cells, organisms and environment water samples, we herein designed and synthesized three organic small molecule fluorescent probes with different recognition sites based on nitrile biphenyl. Through performance comparison, it was found that probe A-HM exhibited the best detection performance for hypochlorite with a low detection limit of 2.47 × 10–6 M. The introduction of hypochlorite will induce probe fluorescence A-HM to turn on, and the fluorescence colour will change from colourless to green. The application of A-HM in biological systems has been demonstrated by the imaging monitoring of hypochlorite in MCF-7, L929 cells and zebrafish. Furthermore, A-HM was also used for the accurate determination of the hypochlorite level in real water samples with high sensitivity and good recoveries.

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The date that support the findings of this study are available from the corresponding author upon reasonable request.

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Acknowledgements

This work was supported by the Natural Science Foundation of Jiangsu Province (No. BK20190862), and China Postdoctoral Science Foundation funded project (No. 2019M651729). We are thankful for Dr. Xu Tang, of Institute for Advanced Materials, Jiangsu university.

Funding

The work was supported by the Natural Science Foundation of Jiangsu Province (No. BK20190862), and China Postdoctoral Science Foundation funded project (No. 2019M651729).

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Authors and Affiliations

  1. Institute for Advanced Materials, School of Materials Science and Engineering, Jiangsu University, Zhenjiang, Jiangsu, 212013, China

    Boxin Zhou, Yunlong Han, Kai Cheng, Mingdong Dong & Xu Tang

  2. College of Computer and Information Engineering, Henan University, Kaifeng, Henan, 475004, China

    Jingyu Liu

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  1. Boxin Zhou
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  2. Yunlong Han
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Contributions

The study conception and design were performed by Boxin Zhou and Xu Tang. Method development and validation were performed by Boxin Zhou and Xu Tang. The first draft of the manuscript was written by Boxin Zhou. All authors read and approved the final manuscript.

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Correspondence to Xu Tang.

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Zhou, B., Han, Y., Liu, J. et al. Design and Synthesis of Novel Fluorescent Probe Based on Cyanobiphenyl and its Application in Detection of Hypochlorite. J Fluoresc 33, 575–586 (2023). https://doi.org/10.1007/s10895-022-03094-y

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