Abstract
The plasma membrane of plant cells is surrounded by a cellulose wall and adjacent cells are connected with a pectin-rich matrix. Disconnection of plant cells and removal of the cell wall experimentally, by either a mechanical or an enzymatic process, results in the production of single, `naked’ plant cells termed `isolated protoplasts’. Protoplasts thus can be considered as special individual plant cells with fully exposed outer plasma membrane which is the only barrier between the external environment and the interior of the living cells. However, plant protoplasts cannot serve as the botanical analog of animal cells, but they should be viewed as injured cells (see Section 2.3.2) that have to go through a repair process before they are capable of sustained division.
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Fehér, A., Dudits, D. (1994). Plant Protoplasts for Cell Fusion and Direct DNA Uptake: Culture and Regeneration Systems. In: Vasil, I.K., Thorpe, T.A. (eds) Plant Cell and Tissue Culture. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-2681-8_4
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