Abstract
We have demonstrated the transfer and expression of a foreign chimeric gene in the grass species, Dactylis glomerata L. This species is a member of the Gramineae sub-family Pooideae, which includes the small grain cereals, from which transformed plants have not yet been obtained. A chimeric hygromycin-resistance gene was introduced into protoplasts isolated from an embryogenic suspension culture, using heat shock followed by electroporation or polyethylene glycol treatment. Cell colonies resistant to 20 μg/ml hygromycin were selected in liquid medium using an agarose bead type culture system. Transformed calli were identified by Southern hybridization. Embryogenic callus was induced to regenerate plants and transformed plants were shown to contain the hygromycin resistance gene.
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Abbreviations
- 2,4-D:
-
2,4-Dichloro-phenoxy-acetic acid
- dicamba:
-
3,6-dichloro-2-methoxy benzoic acid
- PEG:
-
polyethylene glycol
- SDS:
-
Sodium dodecyl sulfate
- SH medium:
-
Medium of Schenk and Hildebrandt (1972)
References
Abdullah R, Cocking EC, Thompson JA (1986) Bio/Technology 4:1087–1090
Cocking EC, Davey MR (1987) Science 236:1259–1262
Dalton SJ (1988) J Plant Physiol 132:170–175 (1988)
Dellaporta S (1983) Maize Genet Coop Newslett 57:26–29
Hauptmann RM, Vasil V, Ozias-Akins P, Tabaeizadeh Z, Rogers SG, Fraley RT, Horsch RB, Vasil IK (1988) Plant Physiol 86:602–604
Heide OM (1987) Physiol. Plant 70:523–529
Horn ME, Conger BV, Harms CT (1988) Plant Cell Rep (in press)
Kao KN, Michayluk MR (1975) Planta 126:105–110
Kyozuka J, Hayashi Y, Shimamoto K (1987) Mol Gen Genet 206:408–413
Lörz H, Baker B, Schell J (1985) Molec Gen Genet 199:178–182
Murray HG, Thompson WF (1980) Nucl Acids Res 8:4321–4325
Negrutiu I, Shillito RD, Potrykus I, Biasini G, Sala F (1987) Plant Mol Biol 8:363–373.
Reed KC, Mann DA (1985) Nucl Acids Res 13:7207–7221
Rhodes CA, Lowe K, Ruby K (1988a) Bio/Technology 6:56–60
Rhodes CA, Pierce DA, Mettler IJ, Mascarenhas D, Detmer JJ (1988b) Science 240:204–207
Rothstein SJ, Lahners KN, Lotstein RJ, Carozzi NB, Jayne SM, Rice DA (1987) Gene 53:153–161
Schenk RU, Hildebrandt AC (1972) Can J Bot 50:199–204
Shillito RD, Paszkowski J, Potrykus I (1983) Plant Cell Rep 2:244–247.
Shillito RD, Saul MW, Paszkowski J, Müller M, Potrykus I (1985) Bio/Technology 3:1099–1103.
Shillito RD, Potrykus I (1987) In: Wu R, Grossman L (eds) Meth Enzymol Vol 153:313–336.
Srinivasan C, Vasil IK (1986) J Plant Physiol 126:41–48
Thompson JA, Abdullah R, Cocking EC (1986) Plant Sci 47:123–133
Toriyama K, Arimoto Y, Uchimiya H, Hinata K (1988) Bio/Technology 6:1072–1074
Vasil IK (1987) J Plant Physiol 128:193–218
Vasil IK (1988) Bio/Technology 6:397–402
Yamada Y, Zhi-Qi Y, Ding-Tai T (1986) Plant Cell Rep 5:85–88
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Communicated by O. L. Gamborg
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Horn, M.E., Shillito, R.D., Conger, B.V. et al. Transgenic plants of Orchardgrass (Dactylis glomerata L.) from protoplasts. Plant Cell Reports 7, 469–472 (1988). https://doi.org/10.1007/BF00272733
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DOI: https://doi.org/10.1007/BF00272733