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Fluorescence Lifetime Imaging (FLIM): Basic Concepts and Recent Applications

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Advanced Time-Correlated Single Photon Counting Applications

Part of the book series: Springer Series in Chemical Physics ((CHEMICAL,volume 111))

Abstract

Fluorescence lifetime imaging (FLIM) is a key fluorescence microscopy technique to map the environment and interaction of fluorescent probes. It can report on photo physical events that are difficult or impossible to observe by fluorescence intensity imaging, because FLIM is independent of the local fluorophore concentration and excitation intensity. A FLIM application relevant for biology concerns the identification of FRET to study protein interactions and conformational changes, and FLIM can also be used to image viscosity, temperature, pH, refractive index and ion and oxygen concentrations, all at the cellular or sub-cellular level, as well as autofluorescence. The basic principles and some recent advances in the application of FLIM, FLIM instrumentation and molecular probe development will be discussed.

An erratum of this chapter can be found under DOI 10.1007/978-3-319-14929-5_21

An erratum to this chapter can be found at http://dx.doi.org/10.1007/978-3-319-14929-5_21

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Notes

  1. 1.

    The Nature milestone website contains a wealth of information on the history and impact of optical microscopy: http://www.nature.com/milestones/milelight/index.html.

  2. 2.

    Free Photochem-CAD software to calculate R 0 from donor and acceptor spectra can be downloaded from http://photochemcad.com [96, 99].

  3. 3.

    Equation (3.5) in [391] should be the same as (3.22) here.

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Acknowledgments

We would like the UK’s MRC, BBSRC and EPSRC for funding. Thanks also to Dylan Owen of the Physics Department and Randall Division of Cell and Molecular Biophysics at King’s College London for valuable comments on the manuscript.

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Authors and Affiliations

  1. Department of Physics, King’s College London, London, WC2R 2LS, UK

    Klaus Suhling

  2. Department of Physics, King’s College London, London, WC2R 2LS, UK

    Liisa M. Hirvonen, James A. Levitt, Pei-Hua Chung, Carolyn Tregido & Alix le Marois

  3. Institute of Neurology, University College London, London, WC1N 3BG, UK

    Dmitri A. Rusakov & Kaiyu Zheng

  4. Randall Division of Cell and Molecular, Biophysics, King’s College London, London, SE1 1UL, UK

    Simon Ameer-Beg, Simon Poland & Simon Coelho

  5. Department of Cancer, Research, Division of Cancer Studies, New Hunt’s, House, Guy’s Campus, King’s College London, London, SE1 1UL, UK

    Richard Dimble

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    Wolfgang Becker

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Suhling, K. et al. (2015). Fluorescence Lifetime Imaging (FLIM): Basic Concepts and Recent Applications. In: Becker, W. (eds) Advanced Time-Correlated Single Photon Counting Applications. Springer Series in Chemical Physics, vol 111. Springer, Cham. https://doi.org/10.1007/978-3-319-14929-5_3

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