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Trichoderma, a Factory of Multipurpose Enzymes: Cloning of Enzymatic Genes

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Fungal Biotechnology and Bioengineering

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

The versatile fungal genus Trichoderma is the most culturable fungus and is industrially exploited at a large scale for the production of several multipurpose enzymes such as pectinase, cellulase, xylanase, chitinase, lipase, protease, amylase, manganese peroxidase, and laccase. This excellent characteristic of synthesizing such a multitude of enzymes ranks this fungus as a magnificent industrial cell factory of enzymes. The present chapter covers the up-to-date information regarding the experimental production of multiple multitask enzymes under different fermentation conditions. The chapter presents a detailed account of potential application of the aforementioned enzymes for different human uses as Trichoderma possesses a highly versatile synthetic machinery producing enzymes for numerous applications. As is well known, these enzymes are widely used in different areas such as production of biofuels, textiles, pulp and paper, food, animal feed, biorefineries, biocontrol, medicine, leather, growth promoters, detergents, fruit juice clarification, antimicrobial purposes, and detoxification of pollutants as well as in waste management. This chapter also explains the biotechnological strategy of gene cloning. In this reference, the important studies have been compiled wherein cloning of the genes responsible for the synthesis of several enzymes has been achieved in bacterial and yeast cells as potent hosts for their successful expression.

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  1. Department of Biotechnology, Faculty of Science, Veer Bahadur Singh Purvanchal University, Jaunpur, Uttar Pradesh, India

    Roshan Lal Gautam & Ram Naraian

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  1. Roshan Lal Gautam
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  1. Department of Genetics Faculty of Agriculture, Beni-Suef University, Beni-Suef, Egypt

    Abd El-Latif Hesham

  2. Department of Botany, Banaras Hindu University, Varanasi, Uttar Pradesh, India

    Ram Sanmukh Upadhyay

  3. Atal Bihari Vajpayee Vishwavidyalaya, Bilaspur, Chhattisgarh, India

    Gauri Dutt Sharma

  4. Department of Botany, Osmania University, Hyderabad, Telangana, India

    Chakravarthula Manoharachary

  5. AgroBioSciences and Chemical & Biochemical Sciences Department, University Mohammed VI Polytechnic (UM6P), Benguerir, Morocco

    Vijai Kumar Gupta

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Gautam, R.L., Naraian, R. (2020). Trichoderma, a Factory of Multipurpose Enzymes: Cloning of Enzymatic Genes. In: Hesham, AL., Upadhyay, R., Sharma, G., Manoharachary, C., Gupta, V. (eds) Fungal Biotechnology and Bioengineering. Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-41870-0_5

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