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Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2

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Abstract

The Trichoderma reesei xln2 gene coding for the pI 9.0 endoxylanase was isolated from the wild-type strain QM6a. The gene contains one intron of 108 nucleotides and codes for a protein of 223 amino acids in which two putative N-glycosylation target sites were found. Three different T. reesei strains were transformed by targeting a construct composed of the xln2 gene, including its promoter, to the endogenous cbh1 locus. Highest overall production levels of xylanase were obtained using T. reesei ALK02721, a genetically engineered strain, as a host. Integration into the cbh1 locus was not required for enhanced expression under control of the xln2 promoter.

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Author notes

  1. K. M. Helena Nevalainen

    Present address: School of Biological Sciences, Macquarie University, 2109, Sydney, N.S.W, Australia

Authors and Affiliations

  1. Research Laboratories, Alko Ltd., P.O. Box 350, SF-00101, Helsinki, Finland

    Ritva Saarelainen, Marja Paloheimo, Richard Fagerström, Pirkko L. Suominen & K. M. Helena Nevalainen

Authors
  1. Ritva Saarelainen
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  2. Marja Paloheimo
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  3. Richard Fagerström
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  4. Pirkko L. Suominen
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  5. K. M. Helena Nevalainen
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Communicated by C. van den Hondel

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Saarelainen, R., Paloheimo, M., Fagerström, R. et al. Cloning, sequencing and enhanced expression of the Trichoderma reesei endoxylanase II (pI 9) gene xln2 . Molec. Gen. Genet. 241, 497–503 (1993). https://doi.org/10.1007/BF00279891

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  • DOI: https://doi.org/10.1007/BF00279891

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