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Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates

  • Bioenergy/Biofuels/Biochemicals
  • Published:
Journal of Industrial Microbiology & Biotechnology

Abstract

The use of glycerol obtained as an intermediate of the biodiesel manufacturing process as carbon source for microbial growth is a potential alternative strategy for the production of enzymes and other high-value bioproducts. This work evaluates the production of cellulase enzymes using glycerol for high cell density growth of Trichoderma harzianum followed by induction with a cellulosic material. Firstly, the influence of the carbon source used in the pre-culture step was investigated in terms of total protein secretion and fungal morphology. Enzymatic productivity was then determined for cultivation strategies using different types and concentrations of carbon source, as well as different feeding procedures (batch and fed-batch). The best strategy for cellulase production was then further studied on a larger scale using a stirred tank bioreactor. The proposed strategy for cellulase production, using glycerol to achieve high cell density growth followed by induction with pretreated sugarcane bagasse, achieved enzymatic activities up to 2.27 ± 0.37 FPU/mL, 106.40 ± 8.87 IU/mL, and 9.04 ± 0.39 IU/mL of cellulase, xylanase, and β-glucosidase, respectively. These values were 2 times higher when compared to the control experiments using glucose instead of glycerol. This novel strategy proved to be a promising approach for improving cellulolytic enzymes production, and could potentially contribute to adding value to biomass within the biofuels sector.

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Acknowledgments

The authors would like to thank the technical staff of the National Laboratory of Science and Technology of Bioethanol (CTBE), and acknowledge the financial support provided by the Brazilian agencies CNPq, CAPES and FAPESP.

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Authors and Affiliations

  1. Brazilian Bioethanol Science and Technology Laboratory, CTBE, Pólo II de Alta Tecnologia, Rua Giuseppe Maximo Scolfaro 10000, Caixa Postal 6192, Campinas, SP, CEP 13083-970, Brazil

    Priscila da Silva Delabona, Deise Juliana Lima, Diogo Robl, Sarita Cândida Rabelo & José Geraldo da Cruz Pradella

  2. Graduate Program of Biotechnology, Federal University of São Carlos, São Carlos, SP, 13565-905, Brazil

    Priscila da Silva Delabona, Cristiane Sanchez Farinas & José Geraldo da Cruz Pradella

  3. Embrapa Instrumentation, Rua XV de Novembro 1452, São Carlos, SP, CEP 13560-970, Brazil

    Cristiane Sanchez Farinas

Authors
  1. Priscila da Silva Delabona
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  2. Deise Juliana Lima
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  3. Diogo Robl
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  4. Sarita Cândida Rabelo
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  5. Cristiane Sanchez Farinas
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  6. José Geraldo da Cruz Pradella
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Correspondence to Priscila da Silva Delabona.

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The financial support provided by the Brazilian agencies CNPq, CAPES and FAPESP and not have any potential Conflicts of interest (financial or non-financial).

This manuscript complies to the Ethical Rules applicable for Journal of Industrial Microbiology and Biotechnology. In this research is not involve human participants and/or animals.

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da Silva Delabona, P., Lima, D.J., Robl, D. et al. Enhanced cellulase production by Trichoderma harzianum by cultivation on glycerol followed by induction on cellulosic substrates. J Ind Microbiol Biotechnol 43, 617–626 (2016). https://doi.org/10.1007/s10295-016-1744-8

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  • DOI: https://doi.org/10.1007/s10295-016-1744-8

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