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Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges

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Synthetic Biology of Cyanobacteria

Part of the book series: Advances in Experimental Medicine and Biology ((AEMB,volume 1080))

Abstract

With the demand for renewable energy growing, hydrogen (H2) is becoming an attractive energy carrier. Developing H2 production technologies with near-net zero carbon emissions is a major challenge for the “H2 economy.” Certain cyanobacteria inherently possess enzymes, nitrogenases, and bidirectional hydrogenases that are capable of H2 evolution using sunlight, making them ideal cell factories for photocatalytic conversion of water to H2. With the advances in synthetic biology, cyanobacteria are currently being developed as a “plug and play” chassis to produce H2. This chapter describes the metabolic pathways involved and the theoretical limits to cyanobacterial H2 production and summarizes the metabolic engineering technologies pursued.

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    Publicly Available Cyanobacterial Databases

    Biological Hydrogen Production Targets (Department of Energy)

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    Acknowledgment

    GCD expresses his gratitude to former students and coworkers whose work provided some of the content summarized herein.

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    Authors and Affiliations

    1. Department of Biochemistry, University of Alberta, Edmonton, AB, Canada

      Anagha Krishnan

    2. Waksman Institute, Rutgers University, Piscataway, NJ, USA

      Xiao Qian, Gennady Ananyev & G. Charles Dismukes

    3. Center for Computational and Integrative Biology, Rutgers, The State University of New Jersey, Camden, NJ, USA

      Desmond S. Lun

    4. Department of Computer Science, Rutgers, The State University of New Jersey, Camden, NJ, USA

      Desmond S. Lun

    5. Department of Plant Biology, Rutgers, The State University of New Jersey, New Brunswick, NJ, USA

      Desmond S. Lun

    6. Department of Chemistry & Chemical Biology, Rutgers University, Piscataway, NJ, USA

      G. Charles Dismukes

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    Correspondence to G. Charles Dismukes .

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    1. School of Chemical Engineering, Tianjin University, Tianjin, China

      Weiwen Zhang

    2. Center for Biosafety Research and Strategy, Tianjin University, Tianjin, China

      Xinyu Song

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    Krishnan, A., Qian, X., Ananyev, G., Lun, D.S., Dismukes, G.C. (2018). Rewiring of Cyanobacterial Metabolism for Hydrogen Production: Synthetic Biology Approaches and Challenges. In: Zhang, W., Song, X. (eds) Synthetic Biology of Cyanobacteria. Advances in Experimental Medicine and Biology, vol 1080. Springer, Singapore. https://doi.org/10.1007/978-981-13-0854-3_8

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