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Imaging Cell Signaling

  • Book
  • © 2024

Overview

Editors:
  1. Christoph Wuelfing

    1. School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

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    You can also search for this editor in PubMed   Google Scholar

  2. Robert F. Murphy

    1. Computational Biology Department, Carnegie Mellon University, Pittsburgh, USA

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2800)

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Table of contents (16 protocols)

  1. Front Matter

    Pages i-xi
    Download chapter PDF

  2. Rapid Preparation of Living Drosophila Pupal Macrophages for Ex Vivo Imaging

    • Tua Karling, Helen Weavers
    Pages 1-10

  3. Generation of 3D Fibroblast-Derived Extracellular Matrix and Analysis of Tumor Cell-Matrix Interactions and Signaling

    • Emily Lay, Tressan Grant, Gernot Walko, Ute Jungwirth
    Pages 11-25

  4. Induced Formation of Plasma Membrane Protrusions with Porous Materials as Instructive Surfaces

    • Morteza Aramesh, Cecilia Persson
    Pages 27-34

  5. Design and Synthesis of DNA Origami Nanostructures to Control TNF Receptor Activation

    • Göktuğ Aba, Ferenc A. Scheeren, Thomas H. Sharp
    Pages 35-53

  6. Controlling the Potency of T Cell Activation Using an Optically Tunable Chimeric Antigen Receptor

    • Muna Fuyal, John R. James
    Pages 55-66

  7. Imaging Cell Signaling in Tissues Using the IBEX Method

    • Yuxuan Meng, Changtai Li, Gregory S. Patient, Yuan Tian, Colin J. Chu
    Pages 67-74

  8. Investigating Epidermal Growth Factor Receptor Trafficking with High-Resolution Enzymatic Protein-Tagging and Transmission Electron Microscopy

    • Nicholas Ariotti
    Pages 75-87

  9. Some Guiding Principles for a “Simple” Correlative Light Electron Microscopy Experiment

    • Elina Mäntylä, Paul Verkade
    Pages 89-102

  10. In Situ Imaging of Proteins Using DNA-PAINT Super-Resolution Microscopy

    • Florian Schueder, Ralf Jungmann
    Pages 103-113

  11. A Magnetic Pincher for the Dynamic Measurement of the Actin Cortex Thickness in Live Cells

    • Joseph Vermeil, Valentin Laplaud, Anumita Jawahar, Dulamkhuu Bujaa, Damien Cuvelier, Julien Heuvingh et al.
    Pages 115-145

  12. Measurement of Forces Acting on Single T-Cell Receptors

    • Lukas Schrangl, Janett Göhring, Florian Kellner, Johannes B. Huppa, Gerhard J. Schütz
    Pages 147-165

  13. A Pipeline for Dynamic Analysis of Mitochondrial Content in Developing T Cells: Bridging the Gap Between High-Throughput Flow Cytometry and Single-Cell Microscopy Analysis

    • Vaibhav Dhyani, Anchi S. Chann, Lopamudra Giri, Sarah M. Russell, Mirren Charnley
    Pages 167-187

  14. Reconstructing Signaling Networks Using Biosensor Barcoding

    • Suyang Wang, Wei-Yu Chi, Gabriel Au, Cheng-Chieh Huang, Jr-Ming Yang, Chuan-Hsiang Huang
    Pages 189-202

  15. Deep Learning-Based Cell Tracking in Deforming Organs and Moving Animals

    • Chentao Wen
    Pages 203-215

  16. Using Dimensionality Reduction to Visualize Phenotypic Changes in High-Throughput Microscopy

    • Alex X. Lu, Alan M. Moses
    Pages 217-229

  17. Learning Morphological, Spatial, and Dynamic Models of Cellular Components

    • Huangqingbo Sun, Robert F. Murphy
    Pages 231-244

  18. Back Matter

    Pages 245-246
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Keywords

About this book

This volume explores recent innovations across the entire pipeline of imaging signal transduction, cell preparation, cellular manipulation, image acquisition, and computational analysis. The chapters in this book cover topics such as rapid preparation of living Drosophila pupal macrophages for ex vivo imaging; controlling the potency of T cell activation using an optically tuneable chimeric antigen receptor; in situ imaging of proteins using DNA-PAINT super-resolution microscopy; reconstructing signaling networks using biosensor barcoding; and morphological, spatial, and dynamic models for cellular components. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and authoritative, Imaging Cell Signaling is a valuable resource that provides guidance to researchers looking to learn how to effectively tailor design projects to image, manipulate, and model signal transduction.



Editors and Affiliations

  • School of Cellular and Molecular Medicine, University of Bristol, Bristol, UK

    Christoph Wuelfing

  • Computational Biology Department, Carnegie Mellon University, Pittsburgh, USA

    Robert F. Murphy

Bibliographic Information

  • Book Title: Imaging Cell Signaling

  • Editors: Christoph Wuelfing, Robert F. Murphy

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-3834-7

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature 2024

  • Hardcover ISBN: 978-1-0716-3833-0Due: 07 June 2024

  • Softcover ISBN: 978-1-0716-3836-1Due: 07 June 2024

  • eBook ISBN: 978-1-0716-3834-7Published: 06 May 2024

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XI, 246

  • Number of Illustrations: 3 b/w illustrations, 73 illustrations in colour

  • Topics: Cell Biology, Materials Science, general

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