Abstract
The study of cell signaling within tissues can be enhanced using highly multiplexed immunohistochemistry to localize the presence and spatial distribution of numerous pathways of interest simultaneously. Additional data can also be gained by placing the identified proteins into the context of adjacent structures, stroma, and interacting partners. Here, we outline a protocol for using the recently described IBEX method on tissues. This is an open and simple cyclic immunohistochemistry approach suited to this application. We describe a simplified protocol and provide guidance on the method, using a 12-marker panel on human retina to demonstrate the approach.
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© 2024 The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature
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Meng, Y., Li, C., Patient, G.S., Tian, Y., Chu, C.J. (2024). Imaging Cell Signaling in Tissues Using the IBEX Method. In: Wuelfing, C., Murphy, R.F. (eds) Imaging Cell Signaling. Methods in Molecular Biology, vol 2800. Humana, New York, NY. https://doi.org/10.1007/978-1-0716-3834-7_6
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DOI: https://doi.org/10.1007/978-1-0716-3834-7_6
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Publisher Name: Humana, New York, NY
Print ISBN: 978-1-0716-3833-0
Online ISBN: 978-1-0716-3834-7
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