© 2016

Estrogen Receptors

Methods and Protocols

  • Kathleen M. Eyster

Part of the Methods in Molecular Biology book series (MIMB, volume 1366)

Table of contents

  1. Front Matter
    Pages i-xvi
  2. Adam E. Handel
    Pages 29-39 Open Access
  3. Meike Wagner, Johannes Jung, Michael Koslowski, Özlem Türeci, Vijay K. Tiwari, Ugur Sahin
    Pages 53-65
  4. Hanquan Liang, Erliang Zeng
    Pages 99-114
  5. Kathleen M. Eyster
    Pages 115-129
  6. Araceli Morales, Raquel Marín, Jorge Marrero-Alonso, Alicia Boto, Mario Díaz
    Pages 163-173
  7. Daniela Fietz, M. Bergmann, K. Hartmann
    Pages 189-205

About this book


This volume examines a wide array of vital technologies for advancing our understanding of the receptor-mediated actions of estrogen. The protocols in this book range from standard methods and vital laboratory workhorses, such as receptor binding assays and western blot, to newer technologies such as RNAseq and proximity ligation assay. Chapters also discuss protocols from a broad range of tissue types to demonstrate the variety of estrogen receptor effects. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Estrogen Receptors: Methods and Protocols is a detailed and helpful resource for scientists who are intrigued by the many facets of estrogen.



RNAseq estrogen receptor genomic nuclear estrogen receptors nongenomic estrogen receptor proximity ligation assay receptor binding assays western blot

Editors and affiliations

  • Kathleen M. Eyster
    • 1
  1. 1.University of South Dakota Sanford School of MedicineVermillionUSA

About the editors

Kathleen (Kathy) Eyster Professor of Physiology and Pharmacology Sanford School of Medicine of the University of South Dakota 605-677-5159

Bibliographic information

  • Book Title Estrogen Receptors
  • Book Subtitle Methods and Protocols
  • Editors Kathleen M. Eyster
  • Series Title Methods in Molecular Biology
  • DOI
  • Copyright Information Springer Science+Business Media New York 2016
  • Publisher Name Humana Press, New York, NY
  • eBook Packages Springer Protocols
  • Hardcover ISBN 978-1-4939-3126-2
  • Softcover ISBN 978-1-4939-5006-5
  • eBook ISBN 978-1-4939-3127-9
  • Series ISSN 1064-3745
  • Series E-ISSN 1940-6029
  • Edition Number 1
  • Number of Pages XVI, 576
  • Number of Illustrations 41 b/w illustrations, 87 illustrations in colour
  • Topics Biochemistry, general
  • Buy this book on publisher's site