Chromatin Immunoprecipitation with Estrogen Receptor 1 and the Promoter of Greb1 in TM4 Sertoli Cells

  • Jing Lin
  • Zhenmin LeiEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1366)


Chromatin immunoprecipitation (ChIP) is a technique to investigate the interaction between proteins and DNA within the natural chromatin context of the cell. There are two previously identified two canonical estrogen response elements (ERE1 and ERE2) present in the 5′-flanking region of the Greb1 gene which is a known estrogen-responsive gene. ChIP results showed the physical interaction between estrogen receptor I (ESR1) and EREs in the Greb1 promoter in TM4 mouse Sertoli cells. This chapter describes the protocol for chromatin immunoprecipitation applied to the estrogen response elements in the Greb1 promoter.

Key words

Chromatin immunoprecipitation Estrogenreceptor Estrogen response element Greb1 Sertoli cell 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of OB/GYN & Women’s HealthUniversity of Louisville School of MedicineLouisvilleUSA
  2. 2.Department of OB/GYN & Women’s HealthUniversity of Louisville Health Sciences CenterLouisvilleUSA

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