Electrophoretic Mobility Shift Assay (EMSA) and Supershift Assay of Cytochrome P450 2B6 in Response to Estrogen

  • Kwi Hye Koh
  • Hyunyoung Jeong
Part of the Methods in Molecular Biology book series (MIMB, volume 1366)


Electrophoretic mobility shift assay (EMSA) is an invaluable tool to study interaction of proteins with DNA. Estrogens are major female hormones and modulate biological function through estrogen receptor (ER). ER regulates its target gene expression via the classical mechanism in which ER directly binds to its target gene promoter or the nonclassical mechanism involving tethering of ER to other transcription factors (such as AP-1 proteins). Here, we describe the EMSA to examine the nonclassical mechanism of ER action in regulation of a gene CYP2B6 by using competition and supershift assays.

Key words

EMSA Supershift Estrogen Estrogenreceptor AP-1 CYP2B6 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Internal MedicineRush University Medical CenterChicagoUSA
  2. 2.Departments of Pharmacy Practice and Biopharmaceutical Sciences, College of PharmacyUniversity of Illinois at ChicagoChicagoUSA

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