Overview
- This book is open access, which means that you have free and unlimited access.
- Includes cutting-edge methods and protocols
- Provides step-by-step detail essential for reproducible results
- Contains key notes and implementation advice from the experts
Part of the book series: Methods in Molecular Biology (MIMB, volume 2533)
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About this book
This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its function, and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five covers modifications that increasethe complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.
Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the increasing importance of in vitro RNA-technologies.
Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the increasing importance of in vitro RNA-technologies.
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Keywords
Table of contents (16 protocols)
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Front Matter
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Ribosome Biogenesis
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Front Matter
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RNA Polymerases
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Front Matter
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Ribosome Assembly, Transport and RNP Complexes
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Front Matter
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Translation
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Front Matter
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Editors and Affiliations
Bibliographic Information
Book Title: Ribosome Biogenesis
Book Subtitle: Methods and Protocols
Editors: Karl-Dieter Entian
Series Title: Methods in Molecular Biology
DOI: https://doi.org/10.1007/978-1-0716-2501-9
Publisher: Humana New York, NY
eBook Packages: Springer Protocols
Copyright Information: The Editor(s) (if applicable) and The Author(s) 2022
Hardcover ISBN: 978-1-0716-2500-2Published: 08 July 2022
Softcover ISBN: 978-1-0716-2503-3Published: 08 July 2022
eBook ISBN: 978-1-0716-2501-9Published: 07 July 2022
Series ISSN: 1064-3745
Series E-ISSN: 1940-6029
Edition Number: 1
Number of Pages: XIV, 287
Number of Illustrations: 11 b/w illustrations, 20 illustrations in colour
Topics: Human Genetics