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  • Open Access
  • © 2022

Ribosome Biogenesis

Methods and Protocols

  • This book is open access, which means that you have free and unlimited access.

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2533)

Buying options

Softcover Book USD 49.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)

Table of contents (16 protocols)

  1. Front Matter

    Pages i-xiv
  2. Ribosome Biogenesis

    1. Front Matter

      Pages 1-1
    2. A Comparative Perspective on Ribosome Biogenesis: Unity and Diversity Across the Tree of Life

      • Michael Jüttner, Sébastien Ferreira-Cerca
      Pages 3-22Open Access
  3. Genomic Organization

    1. Front Matter

      Pages 23-23
    2. Establishment and Maintenance of Open Ribosomal RNA Gene Chromatin States in Eukaryotes

      • Christopher Schächner, Philipp E. Merkl, Michael Pilsl, Katrin Schwank, Kristin Hergert, Sebastian Kruse et al.
      Pages 25-38Open Access
    3. Analysis of Yeast RNAP I Transcription of Nucleosomal Templates In Vitro

      • Philipp E. Merkl, Christopher Schächner, Michael Pilsl, Katrin Schwank, Kristin Hergert, Gernot Längst et al.
      Pages 39-59Open Access
  4. RNA Polymerases

    1. Front Matter

      Pages 61-61
    2. Specialization of RNA Polymerase I in Comparison to Other Nuclear RNA Polymerases of Saccharomyces cerevisiae

      • Philipp E. Merkl, Christopher Schächner, Michael Pilsl, Katrin Schwank, Catharina Schmid, Gernot Längst et al.
      Pages 63-70Open Access
    3. Structural Studies of Eukaryotic RNA Polymerase I Using Cryo-Electron Microscopy

      • Michael Pilsl, Christoph Engel
      Pages 71-80Open Access
    4. Preparation of RNA Polymerase Complexes for Their Analysis by Single-Particle Cryo-Electron Microscopy

      • Michael Pilsl, Florian B. Heiss, Gisela Pöll, Mona Höcherl, Philipp Milkereit, Christoph Engel
      Pages 81-96Open Access
  5. Ribosome Assembly, Transport and RNP Complexes

    1. Front Matter

      Pages 97-97
    2. Eukaryotic Ribosome assembly and Nucleocytoplasmic Transport

      • Michaela Oborská-Oplová, Ute Fischer, Martin Altvater, Vikram Govind Panse
      Pages 99-126Open Access
    3. Tethered MNase Structure Probing as Versatile Technique for Analyzing RNPs Using Tagging Cassettes for Homologous Recombination in Saccharomyces cerevisiae

      • Fabian Teubl, Katrin Schwank, Uli Ohmayer, Joachim Griesenbeck, Herbert Tschochner, Philipp Milkereit
      Pages 127-145Open Access
  6. RNA Modification

    1. Front Matter

      Pages 147-147
    2. Chemical Modifications of Ribosomal RNA

      • Sunny Sharma, Karl-Dieter Entian
      Pages 149-166Open Access
    3. In Vitro Selection of Deoxyribozymes for the Detection of RNA Modifications

      • Anam Liaqat, Maksim V. Sednev, Claudia Höbartner
      Pages 167-179Open Access
    4. Mapping of the Chemical Modifications of rRNAs

      • Jun Yang, Peter Watzinger, Sunny Sharma
      Pages 181-197Open Access
    5. Non-radioactive In Vivo Labeling of RNA with 4-Thiouracil

      • Christina Braun, Robert Knüppel, Jorge Perez-Fernandez, Sébastien Ferreira-Cerca
      Pages 199-213Open Access
  7. Translation

    1. Front Matter

      Pages 215-215
    2. Translation Phases in Eukaryotes

      • Sandra Blanchet, Namit Ranjan
      Pages 217-228Open Access

About this book

This Open Access volume provides comprehensive reviews and describes the latest techniques to study eukaryotic ribosome biogenesis. For more than 50 years ribosomes are a major research topic. Our knowledge about ribosome biogenesis and function such as   transcription, mRNA modification, and translation was the sine qua non for developing the powerful RNA-based vaccines against RNA-viruses causing the world-threatening Covid-19 pandemia. The chapters in this book are organized into six parts. Part One discusses a comparative survey about the unity and diversity of ribosome biogenesis in pro- and eukaryotic cells. Part Two deals with the genomic organization of eukaryotic rDNA and the role of RNA polymerase I in ribosomal RNA transcription. Part Three explores in vitro methods to study RNA polymerase I structure and its function, and Part Four analyzes the nucleo-cytoplasmic transport of assembled ribosomes and RNP complexes. Part Five covers modifications that increase the complexity of rRNAs, and Part Six provides readers with a review of eukaryotic translation and - for the first time - describes a new method to analyze translation in vitro. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Cutting-edge and comprehensive, Ribosome Biogenesis: Methods and Protocols is a valuable resource for scientists and researchers interested in learning more about the increasing importance of in vitro RNA-technologies.

Keywords

  • cryo-electron microscopy
  • ribosome biogenesis
  • subtunit protein composition
  • eukaryotes
  • maturation
  • Open Access

Editors and Affiliations

  • Institute for Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany

    Karl-Dieter Entian

Bibliographic Information

  • Book Title: Ribosome Biogenesis

  • Book Subtitle: Methods and Protocols

  • Editors: Karl-Dieter Entian

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-2501-9

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2022

  • License: CC BY

  • Hardcover ISBN: 978-1-0716-2500-2

  • Softcover ISBN: 978-1-0716-2503-3

  • eBook ISBN: 978-1-0716-2501-9

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 287

  • Number of Illustrations: 11 b/w illustrations, 20 illustrations in colour

  • Topics: Medical Genetics

Buying options

Softcover Book USD 49.99
Price excludes VAT (USA)
Hardcover Book USD 59.99
Price excludes VAT (USA)