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Mitochondrial Medicine

Volume 2: Assessing Mitochondria

  • Book
  • © 2021
  • Latest edition

Overview

Editors:
  1. Volkmar Weissig

    1. Department of Pharmaceutical Sciences, Midwestern University, Glendale, USA

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    You can also search for this editor in PubMed   Google Scholar

  2. Marvin Edeas

    1. Cochin Hospital, Cochin Institute, INSERM U1016, PARIS, France

    View editor publications

    You can also search for this editor in PubMed   Google Scholar

  • Includes cutting-edge methods and protocols
  • Provides step-by-step detail essential for reproducible results
  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2276)

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Table of contents (33 protocols)

  1. Front Matter

    Pages i-xvi
    Download chapter PDF

  2. Mitochondrial Dysfunction in Mitochondrial Medicine: Current Limitations, Pitfalls, and Tomorrow

    • Naig Gueguen, Guy Lenaers, Pascal Reynier, Volkmar Weissig, Marvin Edeas
    Pages 1-29

  3. Preparation of “Functional” Mitochondria: A Challenging Business

    • Stefan Lehr, Sonja Hartwig, Jorg Kotzka
    Pages 31-39

  4. Isolation and Quality Control of Functional Mitochondria

    • Sonja Hartwig, Jorg Kotzka, Stefan Lehr
    Pages 41-55

  5. Purification of Functional Platelet Mitochondria Using a Discontinuous Percoll Gradient

    • Jacob L. Léger, Nicolas Pichaud, Luc H. Boudreau
    Pages 57-66

  6. Mechanical Permeabilization as a New Method for Assessment of Mitochondrial Function in Insect Tissues

    • Alessandro Gaviraghi, Yan Aveiro, Stephanie S. Carvalho, Rodiesley S. Rosa, Matheus P. Oliveira, Marcus F. Oliveira
    Pages 67-85

  7. Analysis of Mitochondrial Retrograde Signaling in Yeast Model Systems

    • Nicoletta Guaragnella, Maša Ždralević, Zdena Palková, Sergio Giannattasio
    Pages 87-102

  8. Native Gel Electrophoresis and Immunoblotting to Analyze Electron Transport Chain Complexes

    • Gisela Beutner, George A. Porter Jr.
    Pages 103-112

  9. Measuring Mitochondrial Hydrogen Peroxide Levels and Glutathione Redox Equilibrium in Drosophila Neuron Subtypes Using Redox-Sensitive Fluorophores and 3D Imaging

    • Lori M. Buhlman, Petros P. Keoseyan, Katherine L. Houlihan, Amber N. Juba
    Pages 113-127

  10. Assessment of Mitochondrial Cell Metabolism by Respiratory Chain Electron Flow Assays

    • Flavia Radogna, Déborah Gérard, Mario Dicato, Marc Diederich
    Pages 129-141

  11. Whole-Cell and Mitochondrial dNTP Pool Quantification from Cells and Tissues

    • Juan C. Landoni, Liya Wang, Anu Suomalainen
    Pages 143-151

  12. Single-Particle Tracking Method in Fluorescence Microscopy to Monitor Bioenergetic Responses of Individual Mitochondria

    • Camille Colin, Emmanuel Suraniti, Emma Abell, Audrey Sémont, Neso Sojic, Philippe Diolez et al.
    Pages 153-163

  13. Investigation of Mitochondrial ADP-Ribosylation Via Immunofluorescence

    • Ann-Katrin Hopp, Michael O. Hottiger
    Pages 165-171

  14. Assessment of Mitochondrial Ca2+ Uptake

    • András T. Deak, Claire Jean-Quartier, Alexander I. Bondarenko, Lukas N. Groschner, Roland Malli, Wolfgang F. Graier et al.
    Pages 173-191

  15. Assessment of Mitochondrial Membrane Potential and NADH Redox State in Acute Brain Slices

    • Andrey Y. Vinokurov, Viktor V. Dremin, Gennadii A. Piavchenko, Olga A. Stelmashchuk, Plamena R. Angelova, Andrey Y. Abramov
    Pages 193-202

  16. Evaluation of Mitochondria Content and Function in Live Cells by Multicolor Flow Cytometric Analysis

    • Hsiu-Han Fan, Tsung-Lin Tsai, Ivan L. Dzhagalov, Chia-Lin Hsu
    Pages 203-213

  17. Analysis of Mitochondrial Dysfunction During Cell Death

    • Vladimir Gogvadze, Boris Zhivotovsky
    Pages 215-225

  18. Modified Blue Native Gel Approach for Analysis of Respiratory Supercomplexes

    • Sergiy M. Nadtochiy, Megan Ngai, Paul S. Brookes
    Pages 227-234

  19. Patch-Clamp Recording of the Activity of Ion Channels in the Inner Mitochondrial Membrane

    • Piotr Bednarczyk, Rafał P. Kampa, Shur Gałecka, Aleksandra Sęk, Agnieszka Walewska, Piotr Koprowski
    Pages 235-248

  20. Assessment of Mitochondrial Protein Glutathionylation as Signaling for CO Pathway

    • Ana S. Almeida, Cláudia Figueiredo-Pereira, Helena L. A. Vieira
    Pages 249-257
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Keywords

About this book

This second edition offers 88 chapters divided among three volumes providing the most comprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine. Volume II guides readers through chapters on mitochondrial dysfunction, functional’ mitochondria, mitochondrial retrograde, mitochondrial dNTP pool quantification, mitochondrial ADP-ribosylation, blue-native gel approach, 3D optical cryo-imaging method, mitochondrial ATP and ROS production, protocol for untargeted metabolomic analysis, and methods for analysis of nitrotyrosine-containing proteins. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls.

 

Authoritative and accessible, Mitochondrial Medicine, Second Edition, Volume 2: Assessing Mitochondria aims to be acomprehensive source of know-how in the wide-ranging field of Mitochondrial Medicine.

Editors and Affiliations

  • Department of Pharmaceutical Sciences, Midwestern University, Glendale, USA

    Volkmar Weissig

  • Cochin Hospital, Cochin Institute, INSERM U1016, PARIS, France

    Marvin Edeas

Bibliographic Information

  • Book Title: Mitochondrial Medicine

  • Book Subtitle: Volume 2: Assessing Mitochondria

  • Editors: Volkmar Weissig, Marvin Edeas

  • Series Title: Methods in Molecular Biology

  • DOI: https://doi.org/10.1007/978-1-0716-1266-8

  • Publisher: Springer New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: Springer Science+Business Media, LLC, part of Springer Nature 2021

  • Hardcover ISBN: 978-1-0716-1265-1Published: 01 June 2021

  • Softcover ISBN: 978-1-0716-1268-2Published: 02 June 2022

  • eBook ISBN: 978-1-0716-1266-8Published: 31 May 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 2

  • Number of Pages: XVI, 459

  • Number of Illustrations: 37 b/w illustrations, 75 illustrations in colour

  • Topics: Biomedicine, general

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