Abstract
ADP-ribosylation is a posttranslational protein modification, involved in various cellular processes, ranging from DNA-damage repair to apoptosis. While its function has been studied amply with respect to genotoxic stress-associated nuclear ADP-ribosylation, the functional relevance of mitochondrial ADP-ribosylation remains so far poorly studied. This is mainly attributed to the absence of powerful techniques able to detect the modification. However, the usage of recently developed anti-ADP-ribose–specific antibodies allows now to investigate mitochondrial ADP-ribosylation under physiological and pathophysiological conditions. In the below method, we describe in detail how to efficiently detect and quantify mitochondrial ADP-ribosylation via immunofluorescence.
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Acknowledgments
The authors would like to thank Tobias Suter (University of Zurich) for providing editorial assistance and critical input during manuscript writing. Work on ADP-ribosylation in the laboratory of M.O.H is supported by the Kanton of Zurich and the Swiss National Science Foundation (SNF 31003A_176177).
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Hopp, AK., Hottiger, M.O. (2021). Investigation of Mitochondrial ADP-Ribosylation Via Immunofluorescence. In: Weissig, V., Edeas, M. (eds) Mitochondrial Medicine . Methods in Molecular Biology, vol 2276. Springer, New York, NY. https://doi.org/10.1007/978-1-0716-1266-8_12
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DOI: https://doi.org/10.1007/978-1-0716-1266-8_12
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