Skip to main content
  • Book
  • Open Access
  • © 2021

Preclinical MRI of the Kidney

Methods and Protocols

  • Includes cutting-edge methods and protocols

  • Provides step-by-step detail essential for reproducible results

  • Contains key notes and implementation advice from the experts

Part of the book series: Methods in Molecular Biology (MIMB, volume 2216)

Buying options

Hardcover Book USD 59.99
Price excludes VAT (USA)

Table of contents (43 protocols)

  1. Front Matter

    Pages i-xiv
  2. Introduction

    1. Front Matter

      Pages 1-1
    2. Recommendations for Preclinical Renal MRI: A Comprehensive Open-Access Protocol Collection to Improve Training, Reproducibility, and Comparability of Studies

      • Andreas Pohlmann, Susan J. Back, Andrea Fekete, Iris Friedli, Stefanie Hectors, Neil Peter Jerome et al.
      Pages 3-23Open Access
  3. Animal Models, Preparation, Monitoring, and Physiological Interventions

    1. Front Matter

      Pages 25-25
    2. Animal Models of Renal Pathophysiology and Disease

      • Adam Hosszu, Tamas Kaucsar, Erdmann Seeliger, Andrea Fekete
      Pages 27-44Open Access
    3. Preparation and Monitoring of Small Animals in Renal MRI

      • Tamas Kaucsar, Adam Hosszu, Erdmann Seeliger, Henning M. Reimann, Andrea Fekete
      Pages 45-55Open Access
    4. Reversible (Patho)Physiologically Relevant Test Interventions: Rationale and Examples

      • Kathleen Cantow, Mechthild Ladwig-Wiegard, Bert Flemming, Andrea Fekete, Adam Hosszu, Erdmann Seeliger
      Pages 57-73Open Access
    5. Preparation of Ex Vivo Rodent Phantoms for Developing, Testing, and Training MR Imaging of the Kidney and Other Organs

      • Jason M. Millward, João S. Periquito, Paula Ramos Delgado, Christian Prinz, Thoralf Niendorf, Sonia Waiczies
      Pages 75-85Open Access
  4. Basic Concepts of Measurement Techniques

    1. Front Matter

      Pages 87-87
    2. Quantitative Assessment of Renal Perfusion and Oxygenation by Invasive Probes: Basic Concepts

      • Kathleen Cantow, Roger G. Evans, Dirk Grosenick, Thomas Gladytz, Thoralf Niendorf, Bert Flemming et al.
      Pages 89-107Open Access
    3. Ultrasound and Photoacoustic Imaging of the Kidney: Basic Concepts and Protocols

      • Sandra Meyer, Dieter Fuchs, Martin Meier
      Pages 109-130Open Access
    4. Hardware Considerations for Preclinical Magnetic Resonance of the Kidney

      • Paula Ramos Delgado, Ekkehard Küstermann, André Kühne, Jason M. Millward, Thoralf Niendorf, Andreas Pohlmann et al.
      Pages 131-155Open Access
    5. MRI Mapping of Renal T1: Basic Concept

      • Stefanie J. Hectors, Philippe Garteiser, Sabrina Doblas, Gwenaël Pagé, Bernard E. Van Beers, John C. Waterton et al.
      Pages 157-169Open Access
    6. MRI Mapping of the Blood Oxygenation Sensitive Parameter T2* in the Kidney: Basic Concept

      • Lu-Ping Li, Bradley Hack, Erdmann Seeliger, Pottumarthi V. Prasad
      Pages 171-185Open Access
    7. Dynamic Contrast Enhancement (DCE) MRI–Derived Renal Perfusion and Filtration: Basic Concepts

      • Michael Pedersen, Pietro Irrera, Walter Dastrù, Frank G. Zöllner, Kevin M. Bennett, Scott C. Beeman et al.
      Pages 205-227Open Access
    8. Noninvasive Renal Perfusion Measurement Using Arterial Spin Labeling (ASL) MRI: Basic Concept

      • Min-Chi Ku, María A. Fernández-Seara, Frank Kober, Thoralf Niendorf
      Pages 229-239Open Access
    9. Renal pH Imaging Using Chemical Exchange Saturation Transfer (CEST) MRI: Basic Concept

      • Dario Livio Longo, Pietro Irrera, Lorena Consolino, Phillip Zhe Sun, Michael T. McMahon
      Pages 241-256Open Access
    10. Sodium (23Na) MRI of the Kidney: Basic Concept

      • James T. Grist, Esben Søvsø Hansen, Frank G. Zöllner, Christoffer Laustsen
      Pages 257-266Open Access
    11. Hyperpolarized Carbon (13C) MRI of the Kidneys: Basic Concept

      • Cornelius von Morze, Galen D. Reed, Zhen J. Wang, Michael A. Ohliger, Christoffer Laustsen
      Pages 267-278Open Access

About this book

This Open Access volume provides readers with an open access protocol collection and wide-ranging recommendations for preclinical renal MRI used in translational research. The chapters in this book are interdisciplinary in nature and bridge the gaps between physics, physiology, and medicine. They are designed to enhance training in renal MRI sciences and improve the reproducibility of renal imaging research. Chapters provide guidance for exploring, using and developing small animal renal MRI in your laboratory as a unique tool for advanced in vivo phenotyping, diagnostic imaging, and research into potential new therapies. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.

Cutting-edge and thorough, Preclinical MRI of the Kidney: Methods and Protocols is a valuable resource and will be of importance to anyone interested in the preclinical aspect of renal and cardiorenal diseases in the fields of physiology, nephrology, radiology, and cardiology. 

This publication is based upon work from COST Action PARENCHIMA, supported by European Cooperation in Science and Technology (COST). COST ( is a funding agency for research and innovation networks. COST Actions help connect research initiatives across Europe and enable scientists to grow their ideas by sharing them with their peers. This boosts their research, career and innovation. PARENCHIMA ( is a community-driven Action in the COST program of the European Union, which unites more than 200 experts in renal MRI from 30 countries with the aim to improve the reproducibility and standardization of renal MRI biomarkers.


  • renal pathophysiology
  • diabetic nephropathy
  • circadian rhythm
  • Magnetic field strength
  • Radio frequency coils
  • Open Access

Editors and Affiliations

  • Berlin Ultrahigh Field Facility (B.U.F.F.), Max Delbrück Center for Molecular Medicine (MDC) in the Helmholtz Association, Berlin, Germany

    Andreas Pohlmann, Thoralf Niendorf

Bibliographic Information

  • Book Title: Preclinical MRI of the Kidney

  • Book Subtitle: Methods and Protocols

  • Editors: Andreas Pohlmann, Thoralf Niendorf

  • Series Title: Methods in Molecular Biology

  • DOI:

  • Publisher: Humana New York, NY

  • eBook Packages: Springer Protocols

  • Copyright Information: The Editor(s) (if applicable) and The Author(s) 2021

  • License: CC BY

  • Hardcover ISBN: 978-1-0716-0977-4Published: 22 January 2021

  • Softcover ISBN: 978-1-0716-0980-4Published: 19 February 2022

  • eBook ISBN: 978-1-0716-0978-1Published: 21 January 2021

  • Series ISSN: 1064-3745

  • Series E-ISSN: 1940-6029

  • Edition Number: 1

  • Number of Pages: XIV, 725

  • Number of Illustrations: 33 b/w illustrations, 169 illustrations in colour

  • Topics: Analytical Chemistry, Biological Techniques, Radiology, Physiology, Biomedical Research

Buying options

Hardcover Book USD 59.99
Price excludes VAT (USA)