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Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation

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Central European Journal of Biology

Abstract

Valerenic acid (VA) is a pharmacologically-active sesquiterpene found in valerian (Valeriana officinalis L., Valerianaceae) roots and rhizomes. The plant produces only small amounts of this metabolite naturally. So, induction of hairy roots as well as elicitation can be useful to increase its commercial production. In this study, Wild-type strain ‘A13’ of Agrobacterium rhizogenes was used to induce hairy roots in valerian. The influence of three different elicitors including Fusarium graminearum extract (FE), methyl jasmonate (MJ) and salicylic acid (SA) on VA production in the selected hairy root line ‘LeVa-C4’ was also investigated. The 23-day-old cultures were treated with different concentrations of the elicitors at exposure time of 3 and 7 days. FE (1%) and MJ (100 µM L−1) highly promoted VA production at 7 days after elicitation, to a level of 12.31- and 6-fold higher than that of non-elicited controls, respectively, and FE did not exert any negative effects on biomass yield of hairy root. SA did not significantly increase the production of VA. This is the first time study to assess the elicitation of hairy root cultures to promote VA biosynthesis in valerian and the resulting experiments demonstrated that F. graminearum extract and MJ were indeed a potent inducer of VA biosynthesis.

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Authors and Affiliations

  1. Department of Biology, Faculty of Science, University of Urmia, P.O. Box 165, Urmia, Iran

    Mohammad Reza Dini Torkamani, Nasser Abbaspour & Reza Heidary

  2. Department of Plant Breeding and Biotechnology, Faculty of Agriculture, University of Urmia, P.O. Box 165, Urmia, Iran

    Morad Jafari

  3. Department of Agricultural Biotechnology, Institute of Biotechnology, University of Urmia, P.O. Box 165, Urmia, Iran

    Morad Jafari

  4. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Naser Safaie

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  1. Mohammad Reza Dini Torkamani
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  2. Morad Jafari
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  5. Naser Safaie
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Correspondence to Morad Jafari.

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Torkamani, M.R.D., Jafari, M., Abbaspour, N. et al. Enhanced production of valerenic acid in hairy root culture of Valeriana officinalis by elicitation. cent.eur.j.biol. 9, 853–863 (2014). https://doi.org/10.2478/s11535-014-0320-3

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  • DOI: https://doi.org/10.2478/s11535-014-0320-3

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