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Increased lignan biosynthesis in the suspension cultures of Linum album by fungal extracts

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Abstract

Linum album accumulates anti-tumor podophyllotoxin (PTOX) and its related lignans, which were originally isolated from an endangered species Podophyllum. In the present study, we examined the effects of five fungal extracts on the production of lignans in L. album cell cultures. Fusarium graminearum extract induced the highest increase of PTOX [143 μg g−1 dry weight (DW) of the L. album cell culture], while Rhizopus stolonifer extract enhanced the accumulation of lariciresinol up to 364 μg g−1 DW, instead of PTOX. Typical elicitors, such as chitin, chitosan, or methyl jasmonate (MeJA), were shown to be less effective in lignan production in L. album cell cultures. These results verified the advantages of fungal extracts to increase lignan production in L. album cell culture, and suggested potential on-demand metabolic engineering of lignan biosynthesis using differential fungal extracts.

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References

  • Baldi A, Jain A, Bisaria VC (2008) Co-culture of arbuscular mycorrhiza-like fungi (Piriformospora indica and Sebacina vermifera) with plant cells of Linum album for enhanced production of podophyllotoxins: a first report. Biotechnol Lett 30:1671–1677

    Article  PubMed  CAS  Google Scholar 

  • Berim A, Spring O, Conrad J, Maitrejean M, Boland W, Petersen M (2005) Enhancement of lignan biosynthesis in suspension cultures of Linum nodiflorum by coronalon, indanoyl-isoleucine and methyl jasmonate. Planta 222:769–776

    Article  PubMed  CAS  Google Scholar 

  • Canel C, Moraes RM, Dayan FE, Ferreira D (2000) Molecules of interest: podophyllotoxin. Phytochemistry 54:115–120

    Article  PubMed  CAS  Google Scholar 

  • Farkya S, Bisaria VS, Sirvastava AK (2004) Biotechnological aspects of the production of the anticancer drug podophyllotoxin. Appl Microbiol Biotechnol 65:504–519

    Article  PubMed  CAS  Google Scholar 

  • Farkya S, Julka A, Mehra R, Datta V, Srivastava AK, Bisaria VS (2005) Enhanced production of secondary metabolites by biotic elicitors in plant cell suspension cultures. In: 5th Asia Pacific Biochemical Engineering Conference. Jeju Island, Korea

  • Federolf K, Alfermann AW, Fuss E (2004) Aryltetralin-lignan formation in two different cell lines of Linum album: deoxypodophyllotoxin 6-hydroxylase, a key enzyme for the formation of 6-methoxypodophyllotoxin. Phytochemistry 68:1397–1406

    Article  Google Scholar 

  • Figgitt DP, Denever SP, Dewick PM, Jackson DE, Willians P (1989) Topoisomerase II: a potential target for novel antifungal agents. Biochem Biophys Res Commun 160:257–262

    Article  PubMed  CAS  Google Scholar 

  • Fuss E (2003) Lignans in plant cell and organ cultures: an overview. Phytochem Rev 2:307–320

    Article  CAS  Google Scholar 

  • Hano C, Addi M, Bensaddek D, Cronier S, Laine E (2006) Differential accumulation of monolignol-derived compounds in elicited flax (Linum usitatissimum) cell suspension cultures. Planta 223:975–989

    Article  PubMed  CAS  Google Scholar 

  • Imre E, Somssich IE, Hahlbrok K (1998) Pathogen defence in plant—a paradigm of biological complexity. Trends Plant Sci 3:86–90

    Article  Google Scholar 

  • Ionkova I (2007) Biotechnological approaches for the production of lignans. Phcog Rev 1:57–68

    CAS  Google Scholar 

  • Muranaka T, Miyata M, Ito K, Tachibana S (1998) Production of podophyllotoxin in Juniperus chinensis callus cultures treated with oligosaccharides and a biogenetic precursor. Phytochemistry 49:491–496

    Article  CAS  Google Scholar 

  • Petersen M, Alfermann AW (2001) The production of cytotoxic lignans by plant cell cultures. Appl Microbiol Biotechnol 55:135–142

    Article  PubMed  CAS  Google Scholar 

  • Van Fürden B, Humburg A, Fuss E (2005) Influence of methyl jasmonate on podophyllotoxin and 6-methoxypodophyllotoxin accumulation in Linum album cell suspension cultures. Plant Cell Rep 24:312–317

    Article  PubMed  Google Scholar 

  • Yousefzadi M, Sharifi M, Behmanesh M, Moyano E, Bonfill M, Cusido RM, Palazon J (2010a) An approach to the biotechnological production of podophyllotoxin: a potential natural product for clinical anticancer drugs. Eng Life Sci 4:281–292

    Article  Google Scholar 

  • Yousefzadi M, Sharifi M, Behmanesh M, Moyano E, Palazon J (2010b) Salicylic acid improves podophyllotoxin production in cell cultures of Linum album by increasing the expression of genes related with its biosynthesis. Biotechnol Lett 32:1739–1743

    Article  PubMed  CAS  Google Scholar 

  • Zhao J, Davis LC, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  PubMed  CAS  Google Scholar 

Download references

Acknowledgments

This work was supported by Tarbiat Modares University and Suntory Foundation for Life Sciences, Bioorganic Research Institute.

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Authors and Affiliations

  1. Department of Plant Biology, Faculty of Biological Sciences, Tarbiat Modares University, 14115-154, Tehran, Iran

    Sedigheh Esmaeilzadeh Bahabadi & Mozafar Sharifi

  2. Department of Plant Pathology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran

    Naser Safaie

  3. Suntory Foundation for Life Sciences, Bioorganic Research Institute, Osaka, Japan

    Jun Murata, Tohru Yamagaki & Honoo Satake

Authors
  1. Sedigheh Esmaeilzadeh Bahabadi
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  2. Mozafar Sharifi
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  3. Naser Safaie
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  4. Jun Murata
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  5. Tohru Yamagaki
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  6. Honoo Satake
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Correspondence to Mozafar Sharifi.

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Bahabadi, S.E., Sharifi, M., Safaie, N. et al. Increased lignan biosynthesis in the suspension cultures of Linum album by fungal extracts. Plant Biotechnol Rep 5, 367–373 (2011). https://doi.org/10.1007/s11816-011-0190-3

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  • DOI: https://doi.org/10.1007/s11816-011-0190-3

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