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Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation

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Abstract

A hairy root culture system of Calotropis gigantea was established and effects of mechanical wounding (MW) and elicitors [methyl jasmonate (MJ), yeast extract (YE) and chitosan (CS)] on cardenolide production were investigated. All treatments stimulated the production of cardenolide in hairy root cultures of C. gigantea. CS was the most effective elicitor, followed by MJ. YE and MW also improved cardenolide yield in individual treatments. The highest cardenolide yield (1,050 ± 55 mg/l) was obtained after adding 50 mg CS/l for 20 days, which was 2.7-fold higher than the control.

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Acknowledgments

This work was supported by the National High Technology Research and Development Program of China (2007AA021501).

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Authors and Affiliations

  1. National Key Laboratory of Biochemical Engineering, Institute of Process Engineering, Chinese Academy of Sciences, Beijing, 100190, China

    Jian Sun, Jie Xiao, Xiaodong Wang, Xiaofan Yuan & Bing Zhao

  2. Graduate University of Chinese Academy of Sciences, Beijing, 100049, China

    Jian Sun & Jie Xiao

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  1. Jian Sun
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  2. Jie Xiao
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  3. Xiaodong Wang
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  4. Xiaofan Yuan
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  5. Bing Zhao
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Correspondence to Bing Zhao.

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Sun, J., Xiao, J., Wang, X. et al. Improved cardenolide production in Calotropis gigantea hairy roots using mechanical wounding and elicitation. Biotechnol Lett 34, 563–569 (2012). https://doi.org/10.1007/s10529-011-0804-4

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  • DOI: https://doi.org/10.1007/s10529-011-0804-4

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