Skip to main content
SpringerLink
Log in

Optimization of elicitors and precursors to enhance valtrate production in adventitious roots of Valeriana amurensis Smir. ex Kom

  • Original Paper
  • Published:
Plant Cell, Tissue and Organ Culture (PCTOC) Aims and scope Submit manuscript

Abstract

Valeriana amurensis Smir. ex Kom. is a traditional Chinese medicinal plant as its roots serve as a rich source of valtrate, a compound with antifungal cytotoxic sedation and tranquilizing activities. In this study, an adventitious root culture of V. amurensis was established, and the effects of different elicitors, including salicylic acid (SA), chitosan (CH), jasmonic acid (JA), methyl jasmonate (MJ), yeast extract (YE), and yeast extract carbohydrate fraction (YECF) along with the precursor citronellal (CI) on production of valepotriates were assayed. MJ and JA highly promoted valtrate production; while, CH moderately promoted valtrate production. In contrast, CI, SA, YE, and YECF were ineffective in promoting valtrate production. Using a response surface methodology, optimal combinations of MJ, JA, and CH were shown to have strong synergistic effects on valtrate production, and this was subsequently confirmed in experimental studies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

Abbreviations

CH:

Chitosan

CI:

Citronellal

2,4-D:

2,4-dichlorophenoxyacetic acid

DW:

Dry weight

FW:

Fresh weight

IBA:

Indole-3-butyric acid

JA:

Jasmonic acid

KT:

Kinetin

MJ:

Methyl jasmonate

SA:

Salicylic acid

YE:

Yeast extract

YECF:

Yeast extract carbohydrate fraction

RSM:

Response surface methodology

References

  • Aerts RJ, Schäfer A, Hesse M, Baumann TW, Slusarenko A (1996) Signalling molecules and the synthesis of alkaloids in Catharanthus roseus seedlings. Phytochemistry 42:417–422

    Article  CAS  Google Scholar 

  • Banerjee S, Rahman L, Uniyal GC, Ahuja PS (1998) Enhanced production of valepotriates by Agrobacterium rhizogenes induced hairy root cultures of Valeriana wallichii DC. Plant Sci 131:203–208

    Article  CAS  Google Scholar 

  • Becker H, Schrall R (1980) Valepotriates in tissue cultures of nine different Valerianaceae species in comparison to literature data of the intact plants. J Nat Prod 43:721–723

    Article  PubMed  CAS  Google Scholar 

  • Biondi S, Fornalé S, Oksman-Caldentey KM, Eeva M, Agostani S, Bagni N (2000) Jasmonates induce over-accumulation of methylputrescine and conjugated polyamines in Hyoscyamus muticus L. root cultures. Plant Cell Rep 19:691–697

    Article  CAS  Google Scholar 

  • Bos R, Woerdenbag HJ, Pras N (2002) Determination of valepotriates. J Chromatogr A 967:131–146

    Article  PubMed  CAS  Google Scholar 

  • Bounthanh C, Bergmann C, Beck JP, Haag-Berrurier M, Anton R (1981) Valepotriates, a new class of cytotoxic and antitumor agents. Planta Med 41:21–28

    Article  PubMed  CAS  Google Scholar 

  • Caetano LC, Charlwood BV, Gahan PB (1999) The localisation and accumulation of valepotriates in hairy roots of Valerianella discoidea (L.) Loisel. Phytochem Anal 10:181–186

    Article  CAS  Google Scholar 

  • Castillo P, Márquez J, Rubluo A, Hernández G, Lara M (2000) Plant regeneration from callus and suspension cultures of Valeriana edulis ssp. procera via simultaneous organogenesis and somatic embryogenesis. Plant Sci 151:115–119

    Article  PubMed  CAS  Google Scholar 

  • Castillo P, Zamilpa A, Márquez J, Hernández G, Lara M, Alvarez L (2002) Comparative study of differentiation levels and valepotriate content of in vitro cultures and regenerated and wild plants of Valeriana edulis ssp. procera. J Nat Prod 65:573–575

    Article  PubMed  CAS  Google Scholar 

  • Chauhan KR, Zhang Q-H, Aldrich JR (2004) Iridodials: enantiospecific synthesis and stereochemical assignment of the pheromone for the golden-eyed lacewing, Chrysopa oculata. Tetrahedron Lett 45:3339–3340

    Article  CAS  Google Scholar 

  • Coste A, Vlase L, Halmagyi A, Deliu C, Coldea G (2011) Effects of plant growth regulators and elicitors on production of secondary metabolites in shoot cultures of Hypericum hirsutum and Hypericum maculatum. Plant Cell Tissue Organ Cult 106:279–288

    Article  CAS  Google Scholar 

  • Cui L, Wang Z-Y, Zhou X-H (2010) Volatile constituents in the roots and rhizomes oils of Valeriana amurensis. J Essent Oil Bear Plant 13:130–134

    CAS  Google Scholar 

  • Dass S, Ramawat KG (2009) Elicitation of guggulsterone production in cell cultures of Commiphora wightii by plant gums. Plant Cell Tissue Organ Cult 96:34–353

    Article  Google Scholar 

  • Frankfater CR, Dowd MK, Triplett BA (2009) Effect of elicitors on the production of gossypol and methylated gossypol in cotton hairy roots. Plant Cell Tissue Organ Cult 98:341–349

    Article  CAS  Google Scholar 

  • Funk C, Brodelius P (1990) Influence of growth regulators and an elicitor on phenylpropanoid metabolism in suspension cultures of Vanilla planifolia. Phytochemistry 29:845–848

    Article  CAS  Google Scholar 

  • Fuzzati N, Wolfender JL, Hostettmann K, Msonthi JD, Mavi S, Molleyres LP (1996) Isolation of antifungal valepotriates from Valeriana capense and the search for valepotriates in crude Valerianaceae extracts. Phytochem Anal 7:76–85

    Article  CAS  Google Scholar 

  • Gränicher F, Christen P, Kapetanidis I (1992) High-yield production of valepotriates by hairy root cultures of Valeriana officnalis L. var. sambucifolia Mikan. Plant Cell Rep 11:339–342

    Article  Google Scholar 

  • Gundlach H, Müller MJ, Kutchan TM, Zenk MH (1992) Jasmonic acid is a signal transducer in elicitor-induced plant cell cultures. Proc Natl Acad Sci 89:2389–2393

    Article  PubMed  CAS  Google Scholar 

  • Hahn MG, Albersheim P (1978) Host-pathogen interactions: XIV. Isolation and partial characterization of an elicitor from yeast extract. Plant Physiol 62:107–111

    Article  PubMed  CAS  Google Scholar 

  • Ignatov A, Clark WG, Cline SD, Psenak M, Krueger RJ, Coscia CJ (1996) Elicitation of dihydrobenzophenanthridine oxidase in Sanguinaria canadensis cell cultures. Phytochemistry 43:1141–1144

    Article  PubMed  CAS  Google Scholar 

  • Karthikeyan RS, Karthikeyan RS, Rakshit SK, Baradarajan A (1996) Optimization of batch fermentation conditions for dextran production. Bioproc Biosyst Eng 15:247–251

    CAS  Google Scholar 

  • Ketchum REB, Gibson DM, Croteau RB, Shuler ML (1999) The kinetics of taxoid accumulation in cell suspension cultures of Taxus following elicitation with methyl jasmonate. Biotechnol Bioeng 62:97–105

    Article  PubMed  CAS  Google Scholar 

  • Kittipongpatana N, Davis DL, Porter JR (2002) Methyl jasmonate increases the production of valepotriates by transformed root cultures of Valerianella locusta. Plant Cell Tissue Organ Cult 71:65–75

    Article  CAS  Google Scholar 

  • Komaraiah P, Amrutha RN, Kishor PBK, Ramakrishna SV (2002) Elicitor enhanced production of plumbagin in suspension cultures of Plumbago rosea L. Enzyme Microb Tech 31:634–639

    Article  CAS  Google Scholar 

  • Korsangruang S, Soonthornchareonnon N, Chintapakorn Y, Saralamp P, Prathanturarug S (2010) Effects of abiotic and biotic elicitors on growth and isoflavonoid accumulation in Pueraria candollei var. candollei and P. candollei var. mirifica cell suspension cultures. Plant Cell Tissue Organ Cult 103:333–342

    Article  CAS  Google Scholar 

  • Lee Y, Lee D-E, Lee H-S, Kim S-K, Lee W-S, Kim S-H, Kim M-W (2011) Influence of auxins, cytokinins, and nitrogen on production of rutin from callus and adventitious roots of the white mulberry tree (Morusalba L.). Plant Cell Tissue Organ Cult 105:9–19

    Article  CAS  Google Scholar 

  • Lenardão EJ, Botteselle GV, Azambuja F, Perin G, Jacob RG (2007) Citronellal as key compound in organic synthesis. Tetrahedron 63:6671–6712

    Article  Google Scholar 

  • Linden JC, Phisalaphong M (2000) Oligosaccharides potentiate methyl jasmonate–induced production of paclitaxel in Taxus canadensis. Plant Sci 158:41–51

    Article  PubMed  CAS  Google Scholar 

  • Liu C, Callow P, Rowland LJ, Hancock JF, Song G-Q (2010) Adventitious shoot regeneration from leaf explants of southern highbush blueberry cultivars. Plant Cell Tissue Organ Cult 103:137–144

    Article  Google Scholar 

  • Luo J, He GY (2004) Optimization of elicitors and precursors for paclitaxel production in cell suspension culture of Taxus chinensis in the presence of nutrient feeding. Process Biochem 39:1073–1079

    Article  CAS  Google Scholar 

  • Maurmann N, Carvalho CMB, Silva LA, Fett-Neto AG, von Poser GL, Rech SB (2006) Valepotriates accumulation in callus, suspended cells and untransformed root cultures of Valeriana glechomifolia. Vitro Cell Dev Biol Plant 42:50–53

    Article  CAS  Google Scholar 

  • Radman R, Saez T, Bucke C, Keshavarz T (2003) Elicitation of plants and microbial cell systems. Biotechnol Appl Biochem 37:91–102

    Article  PubMed  CAS  Google Scholar 

  • Reis RV, Borges APPL, Chierrito TPC et al (2011) Establishment of adventitious root culture of Stevia rebaudiana Bertoni in a roller bottle system. Plant Cell Tissue Organ Cult 106:329–335

    Article  Google Scholar 

  • Salles A, Silva LA, Rech SB, Zanatta N, von Poser GL (2000) Constituents of Valeriana glechomifolia meyer. Biochem Syst Ecol 28:907–910

    Article  PubMed  CAS  Google Scholar 

  • Tanyildizi MS, Özer D, Elibol M (2005) Optimization of α-amylase production by Bacillus sp. using response surface methodology. Process Biochem 40:2291–2296

    Article  CAS  Google Scholar 

  • Violon C, Dekegel D, Vercruysse A (1984) Relation between Valepotriate content and differentiation level in various tissues from Valerianeae. J Nat Prod 47:934–940

    Article  CAS  Google Scholar 

  • Wang W, Zhong J-J (2002) Manipulation of ginsenoside heterogeneity in cell cultures of Panax notoginseng by addition of jasmonates. J Biosci Bioeng 93:48–53

    PubMed  CAS  Google Scholar 

  • Yu K-W, Gao W-Y, Hahn E-J, Paek K-Y (2002) Jasmonic acid improves ginsenoside accumulation in adventitious root culture of Panax ginseng C.A. Meyer. Biochem Eng J 11:211–215

    Article  CAS  Google Scholar 

  • Yuan Y-J, Wei Z-J, Miao Z-Q, Wu J-C (2002) Acting paths of elicitors on Taxol biosynthesis pathway and their synergistic effect. Biochem Eng J 10:77–83

    Article  CAS  Google Scholar 

  • Zhao J, Zhu W-H, Hu Q, Guo Y-Q (2001) Elicitor-induced indole alkaloid biosynthesis in Catharanthus roseus cell culture is related to Ca2+ influx and the oxidative burst. Plant Sci 161:423–431

    Article  CAS  Google Scholar 

  • Zhao J, Lawrence CD, Verpoorte R (2005) Elicitor signal transduction leading to production of plant secondary metabolites. Biotechnol Adv 23:283–333

    Article  PubMed  CAS  Google Scholar 

  • Zhu X-Y, Chai S-J, Chen L-P, Zhang M-F, Yu J-Q (2010) Induction and origin of adventitious roots from chimeras of Brassica juncea and Brassica oleracea. Plant Cell Tissue Organ Cult 101:287–294

    Article  Google Scholar 

Download references

Acknowledgments

This research was supported by Important Tackle Key Problem of Heilongjiang Province (GB05B601-03).

Author information

Authors and Affiliations

  1. Department of Biochemistry, School of Food Science and Engineering, Harbin Institute of Technology, Harbin, 150090, China

    Lei Cui & Zhen-Yu Wang

  2. Forestry College, North-East Forestry University, Harbin, 150040, China

    Zhen-Yu Wang

  3. Bio-Scientific and Technical College, Changchun University, Chang Chun, 130022, China

    Xiu-Hua Zhou

Authors
  1. Lei Cui
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Zhen-Yu Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Xiu-Hua Zhou
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Zhen-Yu Wang.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Cui, L., Wang, ZY. & Zhou, XH. Optimization of elicitors and precursors to enhance valtrate production in adventitious roots of Valeriana amurensis Smir. ex Kom. Plant Cell Tiss Organ Cult 108, 411–420 (2012). https://doi.org/10.1007/s11240-011-0052-2

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11240-011-0052-2

Keywords

Search

Navigation