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Isolation and evaluation of probiotic potential of lactic acid bacteria isolated from poultry intestine

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Abstract

The gut is a source of lactic acid bacteria with remarkable functional and technologies properties as well as a potential source of probiotics. In the present study, 13 strains of Lactobacillus were isolated from poultry intestine and identified according to their 16S rDNA sequences, as well as the evaluation of their probiotic potential. The probiotic properties were tested in aspects of antibiotic susceptibility, antimicrobial activity, exopolysaccharide production, lysozyme tolerance, gut condition tolerance (low pH, bile salt tolerance) and adhesion to human colorectal adenocarcinoma cell line (Caco-2). Most isolates were resistance to streptomycin (10 μg/mL), gentamicin (10 μg/mL), kanamycin (30 μg/mL), penicillin (10 μg/mL) and chloramphenicol (30 μg/mL). Isolates shows strong abilities to adhere to Caco-2 cell in the range of (76 to 85%). Isolates SHA101 to SHA113 showed high survival rate under gastrointestinal tract condition (>80%), indicating their potential in application of probiotics. The results of these tests indicate that the lactic acid bacteria isolated from poultry intestine have potential use as probiotic in various products.

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References

  • Ahmed, F.E., Genetically modified probiotics in foods, Trends Biotechnol., 2003, vol. 21, no. 11, pp. 491–497. doi 10.1016/j.tibtech.2003.09.006

    Article  CAS  PubMed  Google Scholar 

  • Bian, L., Molan, A.-L., Maddox, I., and Shu, Q., Antimicrobial activity of Lactobacillus reuteri DPC16 supernatants against selected food borne pathogens, World J. Microbiol. Biotechnol., 2010, vol. 27, no. 4, pp. 991–998. doi 10.1007/s11274-010-0543-z

    Article  Google Scholar 

  • Blajman, J., Gaziano, C., Zbrun, M.V., Soto, L., Astesana, D., Berisvil, A., Scharpen, A.R., Signorini, M., and Frizzo, L., In vitro and in vivo screening of native lactic acid bacteria toward their selection as a probiotic in broiler chickens, Res. Vet. Sci., 2015, vol. 101, pp. 50–56. doi 10.1016/j.rvsc.2015.05.017

    Article  PubMed  Google Scholar 

  • Caggia, C., De Angelis, M., Pitino, I., Pino, A., and Randazzo, C., Probiotic features of Lactobacillus strains isolated from Ragusano and Pecorino Siciliano cheeses, Food Microbiol., 2015, vol. 50, pp. 109–117.

    Article  CAS  PubMed  Google Scholar 

  • Chang, L., Zhang, Z.-Y., Ke, D., Jian-Ping, Y., and Xiao-Kui, G., Antibiotic resistance of probiotic strains of lactic acid bacteria isolated from marketed foods and drugs, Biomed. Environ. Sci., 2009, vol. 22, no. 5, pp. 401–412.

    Article  Google Scholar 

  • CLSI, Performance Standards for Antimicrobial Susceptibility Testing: Twenty-Forth Informational Supplement 34: M100-S124, 2014.

  • Collado, M.C., Gueimonde, M., Sanz, Y., and Salminen, S., Adhesion of selected Bifidobacterium strains to human intestinal mucus and the role of adhesion in enteropathogen exclusion, J. Food Prot., 2005, vol. 68, no. 12, pp. 2672–2678.

    Article  PubMed  Google Scholar 

  • Collado, M.C., Jalonen, L., Meriluoto, J., and Salminen, S., Protection mechanism of probiotic combination against human pathogens: in vitro adhesion to human intestinal mucus, Asia Pacific J. Clin. Nutr., 2006, vol. 15, no. 4, pp. 570–575.

    Google Scholar 

  • De Palencia, P.F., López, P., Corbí, A.L., Peláez, C., and Requena, T., Probiotic strains: survival under simulated gastrointestinal conditions, in vitro adhesion to Caco-2 cells and effect on cytokine secretion, Eur. Food Res. Technol., 2008, vol. 227, no. 5, pp. 1475–1484.

    Article  Google Scholar 

  • Degeest, B., Janssens, B., and De Vuyst, L., Exopolysaccharide (EPS) biosynthesis by Lactobacillus sakei 0–1: production kinetics, enzyme activities and EPS yields, J. Appl. Microbiol., 2001, vol. 91, pp. 470–477.

    Article  CAS  PubMed  Google Scholar 

  • Dias, R., Vilas-Boas, E., Campos, F.M., Hogg, T., and Couto, J.A., Activity of lysozyme on Lactobacillus hilgardii strains isolated from Port wine, Food Microbiol., 2015, vol. 49, pp. 6–11.

    Article  CAS  PubMed  Google Scholar 

  • Domingos-Lopes, M.F., Stanton, C., Ross, P.R., Dapkevicius, M.L., and Silva, C.C., Genetic diversity, safety and technological characterization of lactic acid bacteria isolated from artisanal Pico cheese, Food Microbiol., 2017, vol. 63, pp. 178–190. doi 10.1016/j.fm.2016.11.014

    Article  CAS  PubMed  Google Scholar 

  • Drago, L., Rodighiero, V., Mattina, R., Toscano, M., and De Vecchi, E., In vitro selection of antibiotic resistance in the probiotic strain Lactobacillus rhamnosus GG ATCC 53103, J. Chemother., 2013, vol. 4, pp. 211–215.

    Google Scholar 

  • FAO/WHO, Evaluation of health and nutritional properties of probiotics in food including powder milk with live lactic acid bacteria. Expert consultation report. Food and Agriculture Organization of the United Nations and World Health Organization, Córdoba, Argentina (1–4 October 2001).

  • Fontana, C., Cocconcelli, P.S., Vignolo, G., and Saavedra, L., Occurrence of antilisterial structural bacteriocins genes in meat borne lactic acid bacteria, Food Control, 2015, vol. 47, pp. 53–59.

    Article  CAS  Google Scholar 

  • Garcia-Hernandez, Y., Perez-Sanchez, T., Boucourt, R., Balcazar, J.L., Nicoli, J.R., Moreira-Silva, J., Rodriguez, Z., Fuertes, H., Nunez, O., Albelo, N., and Halaihel, N. Isolation, characterization and evaluation of probiotic lactic acid bacteria for potential use in animal production, Res. Vet. Sci., 2016, vol. 108, pp. 125–132. doi 10.1016/j.rvsc.2016.08.009

    Article  CAS  PubMed  Google Scholar 

  • Handa, S. and Sharma, N., In vitro probiotic potential of Lactobacillus delbreuckii subsp. bulgaricus F18 isolated from homemade butter, Microbiology (Moscow), 2017, vol. 86, no. 1, pp. 143–149. doi 10.1134/s0026261716060114

    Article  CAS  Google Scholar 

  • Kaewiad, K., Kaewnopparat, S., and Kaewnopparat, N., In vitro comparison of probiotic properties of Lactobacillus fermentum SK54 isolated from new born baby with Lactobacillus rhamnosus GG ATCC 53103, Adv. Mat. Res., 2015, vol. 1060, pp. 215–218.

    Google Scholar 

  • Kandola, S., Kumar, R., Mishra, A.K., and Singh, A., Investigation of acid tolerance attribute of various Lactobacillus casei group strains, Ind. J. Animal Res., 2016, vol. 50, no. 2, pp. 190–193.

    Google Scholar 

  • Lavilla-Lerma, L., Pérez-Pulido, R., Martínez-Bueno, M., Maqueda, M., and Valdivia, E., Characterization of functional, safety, and gut survival related characteristics of Lactobacillus strains isolated from farmhouse goat’s milk cheeses, Int. J. Food Microbiol., 2013, vol. 163, no. 2, pp. 136–145.

    Article  CAS  PubMed  Google Scholar 

  • Lee, Y.-K., Puong, K.-Y., Ouwehand, A.C., and Salminen, S., Displacement of bacterial pathogens from mucus and Caco-2 cell surface by lactobacilli, J. Med. Microbiol., 2003, vol. 52, no. 10, pp. 925–930.

    Article  PubMed  Google Scholar 

  • Liu, C.F., Tseng, K.C., Chiang, S.S., Lee, B.H., Hsu, W.H., and Pan, T.M., Immunomodulatory and antioxidant potential of Lactobacillus exopolysaccharides, J. Sci. Food Agricul., 2011, vol. 91, no. 12, pp. 2284–2291.

    CAS  Google Scholar 

  • Mandal, H., Jariwala, R., and Bagchi, T., Isolation and characterization of lactobacilli from human faeces and indigenous fermented foods for their potential application as probiotics, Canad. J. Microbiol., 2015, vol. 62, no. 4, pp. 349–359.

    Article  Google Scholar 

  • Manini, F., Casiraghi, M., Poutanen, K., Brasca, M., Erba, D., and Plumed-Ferrer, C., Characterization of lactic acid bacteria isolated from wheat bran sourdough, LWTFood Sci. Technol., 2016, vol. 66, pp. 275–283.

    CAS  Google Scholar 

  • Minervini, F., De Angelis, M., Surico, R., Di Cagno, R., Gänzle, M., and Gobbetti, M., Highly efficient synthesis of exopolysaccharides by Lactobacillus curvatus DPPMA10 during growth in hydrolyzed wheat flour agar, Int. J. Food Microbiol., 2010, vol. 141, no. 1, pp. 130–135.

    Article  CAS  PubMed  Google Scholar 

  • Oh, Y.J., and Jung, D.S., Evaluation of probiotic properties of Lactobacillus and Pediococcus strains isolated from Omegisool, a traditionally fermented millet alcoholic beverage in Korea, LWT-Food Sci. Technol., 2015, vol. 63, no. 1, pp. 437–444.

    Article  CAS  Google Scholar 

  • Panel, E.F., Guidance on the assessment of bacterial susceptibility to antimicrobials of human and veterinary importance, EFSA J., 2012, vol. 10, no. 6, p. 2740.

    Google Scholar 

  • Pingitore, E.V., Pessione, A., Fontana, C., Mazzoli, R., and Pessione, E., Comparative proteomic analyses for elucidating metabolic changes during EPS production under different fermentation temperatures by Lactobacillus plantarum Q823, Int. J. Food Microbiol., 2016, vol. 238, pp. 96–102.

    Article  Google Scholar 

  • Rendueles, O., Kaplan, J.B., and Ghigo, J.M., Antibiofilm polysaccharides, Environ. Microbiol., 2013, vol. 15, no. 2, pp. 334–346.

    Article  CAS  PubMed  Google Scholar 

  • Riaz Rajoka, M.S., Shi, J., Zhu, J., Shao, D., Huang, Q., Yang, H., and Jin, M., Capacity of lactic acid bacteria in immunity enhancement and cancer prevention, Appl. Microbiol. Biotechnol., 2017, vol. 101, no. 1, pp. 35–45. doi 10.1007/s00253-016-8005-7

    Article  CAS  PubMed  Google Scholar 

  • Russo, P., López, P., Capozzi, V., De Palencia, P.F., Dueñas, M.T., Spano, G., and Fiocco, D., Beta-glucans improve growth, viability and colonization of probiotic microorganisms, Int. J. Mol. Sci., 2012, vol. 13, no. 5, pp. 6026–6039.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Saitou, N. and Nei, M., The neighbor-joining method: a new method for reconstructing phylogenetic trees, Mol. Biol. Evol., 1987, vol. 4, no. 4, pp. 406–425.

    CAS  PubMed  Google Scholar 

  • Salminen, S., von Wright, A., Morelli, L., Marteau, P., Brassart, D., de Vos, W.M., Fondén, R., Saxelin, M., Collins, K., and Mogensen, G., Demonstration of safety of probiotics—a review, Int. J. Food Microbiol., 1998, vol. 44, no. 1, pp. 93–106.

    Article  CAS  PubMed  Google Scholar 

  • Schiffrin, E., Rochat, F., Link-Amster, H., Aeschlimann, J., and Donnet-Hughes, A., Immunomodulation of human blood cells following the ingestion of lactic acid bacteria, J. Dairy Sci., 1995, vol. 78, no. 3, pp. 491–497.

    Article  CAS  PubMed  Google Scholar 

  • Shahid Riaz, M., Shaheen, T., Batool, N., Saleem, S., and Hayat, F., Lactic acid bacteria as probiotic candidate and their application, J. Biol. Today’s World, 2015, vol. 4, no. 12, pp. 209–216.

    Google Scholar 

  • Solieri, L., Bianchi, A., Mottolese, G., Lemmetti, F., and Giudici, P., Tailoring the probiotic potential of non-starter Lactobacillus strains from ripened Parmigiano Reggiano cheese by in vitro screening and principal component analysis, Food Microbiol., 2014, vol. 38, pp. 240–249.

    Article  CAS  PubMed  Google Scholar 

  • Tamura, K., Nei, M., and Kumar, S., Prospects for inferring very large phylogenies by using the neighbor-joining method, Proc. Natl. Acad. Sci. U. S. A., 2004, vol. 101, no. 30, pp. 11030–11035.

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  • Tavakoli, M., Hamidi-Esfahani, Z., Hejazi, M.A., Azizi, M.H., and Abbasi, S., Characterization of probiotic abilities of lactobacilli isolated from Iranian koozeh traditional cheese, Polish J. Food Nutr. Sci., 2017, vol. 67, no. 1. doi 10.1515/pjfns-2016-0003

    Google Scholar 

  • Tejero-Sariñena, S., Barlow, J., Costabile, A., Gibson, G.R., and Rowland, I., In vitro evaluation of the antimicrobial activity of a range of probiotics against pathogens: evidence for the effects of organic acids, Anaerobe, 2012, vol. 18, no. 5, pp. 530–538.

    Article  PubMed  Google Scholar 

  • Todorov, S.D., Leblanc, J.G., and Franco, B.D., Evaluation of the probiotic potential and effect of encapsulation on survival for Lactobacillus plantarum ST16Pa isolated from papaya, World J. Microbiol. Biotechnol., 2012, vol. 28, no. 3, pp. 973–984. doi 10.1007/s11274-011-0895-z

    Article  CAS  PubMed  Google Scholar 

  • Turchi, B., Mancini, S., Fratini, F., Pedonese, F., Nuvoloni, R., Bertelloni, F., Ebani, V.V., and Cerri, D., Preliminary evaluation of probiotic potential of Lactobacillus plantarum strains isolated from Italian food products, World J. Microbiol. Biotechnol., 2013, vol. 29, no. 10, pp. 1913–1922.

    Article  PubMed  Google Scholar 

  • Verdenelli, M.C., Ghelfi, F., Silvi, S., Orpianesi, C., Cecchini, C., and Cresci, A., Probiotic properties of Lactobacillus rhamnosus and Lactobacillus paracasei isolated from human faeces, Eur. J. Nutr., 2009, vol. 48, no. 6, pp. 355–363.

    Article  PubMed  Google Scholar 

  • Vesterlund, S., Paltta, J., Karp, M., and Ouwehand, A.C., Measurement of bacterial adhesion—in vitro evaluation of different methods, J. Microbiol. Methods, 2005, vol. 60, no. 2, pp. 225–233.

    Article  CAS  PubMed  Google Scholar 

  • Yu, Z., Zhang, X., Li, S., Li, C., Li, D., and Yang, Z., Evaluation of probiotic properties of Lactobacillus plantarum strains isolated from Chinese sauerkraut, World J. Microbiol. Biotechnol., 2013, vol. 29, no. 3, pp. 489–498. doi 10.1007/s11274-012-1202-3

    Article  CAS  PubMed  Google Scholar 

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Authors and Affiliations

  1. Key Laboratory for Space Bioscience and Space Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi’an, Shaanxi, 710072, People’s Republic of China

    M. Sh. R. Rajoka, F. Ahmad & J. Shi

  2. Institute of Microbiology, University of Agriculture Faisalabad, Punjab, 38000, Pakistan

    H. F. Hayat, S. Sarwar & S. Z. H. Shah

  3. Institute of Biotechnology, University of Agriculture Faisalabad, Punjab, 38000, Pakistan

    H. M. Mehwish, N. Hussain & M. Khurshid

  4. Department of Neonatology, The First Affiliated Hospital, College of Medicine, Xi’an, Shaanxi, 710072, People’s Republic of China

    M. Siddiqu

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  1. M. Sh. R. Rajoka
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  2. H. F. Hayat
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  3. S. Sarwar
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  4. H. M. Mehwish
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  5. F. Ahmad
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  6. N. Hussain
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  7. S. Z. H. Shah
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  8. M. Khurshid
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Correspondence to M. Sh. R. Rajoka.

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Rajoka, M.S.R., Hayat, H.F., Sarwar, S. et al. Isolation and evaluation of probiotic potential of lactic acid bacteria isolated from poultry intestine. Microbiology 87, 116–126 (2018). https://doi.org/10.1134/S0026261718010150

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