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Evaluation of the probiotic potential and effect of encapsulation on survival for Lactobacillus plantarum ST16Pa isolated from papaya

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Abstract

Capability to produce antilisterial bacteriocins by lactic acid bacteria (LAB) can be explored by the food industry as a tool to increase the safety of foods. Furthermore, probiotic activity of bacteriogenic LAB brings extra advantages to these strains, as they can confer health benefits to the consumer. Beneficial effects depend on the ability of the probiotic strains to maintain viability in the food during shelf-life and to survive the natural defenses of the host and multiply in the gastrointestinal tract (GIT). This study evaluated the probiotic potential of a bacteriocinogenic Lactobacillus plantarum strain (Lb. plantarum ST16Pa) isolated from papaya fruit and studied the effect of encapsulation in alginate on survival in conditions simulating the human GIT. Good growth of Lb. plantarum ST16Pa was recorded in MRS broth with initial pH values between 5.0 and 9.0 and good capability to survive in pH 4.0, 11.0 and 13.0. Lb. plantarum ST16Pa grew well in the presence of oxbile at concentrations ranging from 0.2 to 3.0%. The level of auto-aggregation was 37%, and various degrees of co-aggregation were observed with different strains of Lb. plantarum, Enterococcus spp., Lb. sakei and Listeria, which are important features for probiotic activity. Growth was affected negatively by several medicaments used for human therapy, mainly anti-inflammatory drugs and antibiotics. Adhesion to Caco-2 cells was within the range reported for other probiotic strains, and PCR analysis indicated that the strain harbored the adhesion genes mapA, mub and EF-Tu. Encapsulation in 2, 3 and 4% alginate protected the cells from exposure to 1 or 2% oxbile added to MRS broth. Studies in a model simulating the transit through the GIT indicated that encapsulated cells were protected from the acidic conditions in the stomach but were less resistant when in conditions simulating the duodenum, jejunum, ileum and first section of the colon. To our knowledge, this is the first report on a bacteriocinogenic LAB isolated from papaya that presents application in food biopreservation and may be beneficial to the consumer health due to its potential probiotic characteristics.

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Acknowledgments

The authors would like to thank Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES, Brasil), Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq, Brasil), and Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET, Argentina) for financial support.

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Authors and Affiliations

  1. Departamento de Alimentos e Nutrição Experimental, Faculdade de Ciências Farmacêuticas, Laboratório de Microbiologia de Alimentos, Universidade de São Paulo, Av. Prof. Lineu Prestes 580 Bloco 14, São Paulo, SP, 05508-000, Brazil

    Svetoslav D. Todorov & Bernadette D. G. M. Franco

  2. Centro de Referencia Para Lactobacilos (CERELA-CONICET), Chacabuco 145, C.P. T4000ILC, San Miguel de Tucumán, Tucumán, Argentina

    Jean Guy LeBlanc

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  1. Svetoslav D. Todorov
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  2. Jean Guy LeBlanc
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  3. Bernadette D. G. M. Franco
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Correspondence to Svetoslav D. Todorov.

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Todorov, S.D., LeBlanc, J.G. & Franco, B.D.G.M. Evaluation of the probiotic potential and effect of encapsulation on survival for Lactobacillus plantarum ST16Pa isolated from papaya. World J Microbiol Biotechnol 28, 973–984 (2012). https://doi.org/10.1007/s11274-011-0895-z

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