, 104:79 | Cite as

Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp. pekinensis)

  • Etsuo Matsumoto
  • Chika Yasui
  • Michio Ohi
  • Motohisa Tsukada


A restriction fragment length polymorphism (RFLP) – based linkage map of Chinese cabbage (Brassica rapa ssp. pekinensis) (2n=20) including two agronomic traits, clubroot resistance and orange-yellow pigmentation, was constructed using doubled haploid parents. The total linkage distance was 735 cM; 63 loci were distributed into ten linkage groups. Clubroot resistance of the parental line T136-8 to the current pathotype, race 2, was predominantly controlled by a single dominant gene that originated from European turnip. The locus for clubroot resistance by the dominant major gene (CRa) was mapped on linkage group 3, and RFLP loci HC352b and HC181 were located 3 cM and 12 cM from it, respectively. The locus HC352b was identified by a 4.4 Kb Eco R I fragment, which segregated for null allele. The absence of an allelic fragment in HC352b could be interpreted by deletion in the resistance source; homozygotes for CRa could be efficiently selected by detecting null types for the marker. Orange-yellow pigmentation expressed in head inner leaves and petals was governed by a single recessive gene. The locus (Oy) for the pigmentation was mapped on linkage group 1, being located 17–19 cM from three RFLP loci that were closely linked to each other. The linkage analysis for clubroot resistance and unique pigmentation revealed some informative RFLP markers. Identification of molecular markers for clubroot resistance and other agronomically important traits would provide useful information in breeding programs of Chinese cabbage.

Chinese cabbage clubroot resistance linkage map orange-yellow pigmentation Plasmodiophora brassicae RFLP markers 


  1. Buczacki, S.T., H. Toxopeus, P. Mattusch, T.D. Johnston, G.R. Dixon & L.A. Hobolth, 1975. Study of physiologic specialization in Plasmodiophora brassicae: proposals for attempted rationalization through an international approach. Trans Br Mycol Soc 65: 295-303.CrossRefGoogle Scholar
  2. Chyi, Y.S., M.E. Hoenecke & J.L. Sernyk, 1992. A genetic linkage map of restriction fragment length polymorphism loci for Brassica rapa(syn. campestris). Genome 35: 746-757.Google Scholar
  3. Crisp, P., I.R. Crute, R.A. Sutherland, S.M. Angell, K. Bloor, H. Burgess and P.L. Gordon, 1989. The exploitation of genetic resources of Brassica oleracea in breeding for resistance to clubroot (Plasmodiophora brassicae). Euphytica 42: 215-226.Google Scholar
  4. Crute, I.R., A.R. Gray, P. Crisp & S.T. Buczacki, 1980. Variation in Plasmodiophora brassicaeand resistance to clubroot disease in Brassicasand allied crops - a critical review. Plant Breed Abstr 50: 91-104.Google Scholar
  5. Ferreira, M.E., P.H. Williams & T.C. Osborn, 1994. RFLP mapping of Brassica napususing doubled haploid lines. Theor Appl Genet 89: 615-621.CrossRefGoogle Scholar
  6. Figdore, S.S., W.C. Kennard, K.M. Song M.K. Slocum & T.C. Osborn, 1988. Assessment of the degree of restriction fragment length polymorphism in Brassica. Theor Appl Genet 75: 833-840.Google Scholar
  7. Figdore, S.S., M.E. Ferreira, M.K. Slocum & P.H. Williams, 1993. Association of RFLP markers with trait loci affecting clubroot resistance and morphological characters in Brassica oleraceaL. Euphytica 69: 33-44.CrossRefGoogle Scholar
  8. Grandclement, C. & G. Thomas, 1996. Detection and analysis of QTLs based on RAPD markers for polygenic resistance to Plasmodiophora brassicaeWoron in Brassica oleraceaL. Theor Appl Genet 93: 86-90.CrossRefGoogle Scholar
  9. Gubler, U. & J. Hoffman, 1983. A simple and very efficient method for generating cDNA libraries. Gene 25: 263-269.PubMedCrossRefGoogle Scholar
  10. James, R.V. & P.H. Williams, 1980. Clubroot resistance and linkage in Brassica campestris. Phytopathology 70: 776-779.CrossRefGoogle Scholar
  11. Kianian, S.F. & C.F. Quiros, 1992. Generation of a Brassica oleraceacomposite RFLPmap: linkage arrangements among various populations and evolutionary implications. Theor Appl Genet 84: 544-554.CrossRefGoogle Scholar
  12. Kosambi, D.D., 1944. The estimation of map distances from recombination values. Ann Eugen 12: 172-175.Google Scholar
  13. Lander, E.S., P. Green, J. Abrahamson, A. Barlow, M.J. Daly, S.E. Lincoln & L. Newburg, 1987. MAPMAKER: an interactive computer package for constructing primary genetic linkage maps of experimental and natural populations. Genomics 1: 174-181.PubMedCrossRefGoogle Scholar
  14. Landry, B.S., N. Hubert, T. Etoh, J.J. Harada & S.E. Lincoln, 1991. A genetic map for Brassica napusbased on restriction fragment length polymorphisms detected with expressed DNA sequences. Genome 34: 543-552.Google Scholar
  15. Landry, B.S., N. Hubert, R. Crete, M.S. Chang, S.E. Lincoln & T. Etoh, 1992. A genetic map for Brassica oleraceabased on RFLP markers detected with expressed DNA sequences and mapping of resistance genes to race 2 of Plasmodiophora brassicae(Woronin). Genome 35: 409-420.Google Scholar
  16. McGrath, J.M. & C.F. Quiros, 1991. Inheritance of isozyme and RFLP markers in Brassica campestrisand comparison with B. oleracea. Theor Appl Genet 82: 668-673.CrossRefGoogle Scholar
  17. Murray, M. & W.F. Thompson, 1980. Rapid isolation of high molecular weight plant DNA. Nucl Acid Res 8: 4321-4325.Google Scholar
  18. Reed, K.C. & D.A. Mann, 1985. Rapid transfer of DNA from agarose gels to nylon membranes. Nucl Acid Res 13: 7207-7221.Google Scholar
  19. Rogers, S.O. & A.J. Bendich, 1985. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol 5: 69-76.CrossRefGoogle Scholar
  20. Seaman, W.L., J.C. Walker & R.H. Larson, 1963. A new race of Plasmodiophora brassicaeaffecting Badger Shipper cabbage. Phytopathology 53: 1426-1429.Google Scholar
  21. Slocum, M.K., S.S. Figdore, W.C. Kennard, J.Y. Suzuki & T.C. Osborn, 1990. Linkage arrangement of restriction fragment length polymorphism loci in Brassica oleracea. Theor Appl Genet 80: 57-64.CrossRefGoogle Scholar
  22. Song, K.M., J.Y. Suzuki, M.K. Slocum, P.H. Williams & T.C. Osborn, 1991. A linkage map of Brassica rapa(syn. campestris) based on restriction fragment length polymorphism loci. Theor Appl Genet 82: 296-304.CrossRefGoogle Scholar
  23. Staub, J.E., F.C. Serquen & M. Gupta, 1996. Genetic markers, map construction, and their application in plant breeding. Hort Science 31: 729-741.Google Scholar
  24. Teutonico, R.A. & T.C. Osborn, 1994. Mapping of RFLP and qualitative trait loci in Brassica rapaand comparison to the linkage maps of B. napus, B. oleracea, and Arabidopsis thaliana. Theor Appl Genet 89: 885-894.CrossRefGoogle Scholar
  25. Tjallingii, F., 1965. Testing clubroot-resistance of turnips in the Netherlands and the physiologic specialization of Plasmodiophora brassicae. Euphytica 14: 1-22.Google Scholar
  26. Toxopeus, H. & A.M.P. Janssen, 1975. Clubroot resistance in turnip II. The slurry screening method and clubroot races in the Netherlands. Euphytica 24: 751-755.CrossRefGoogle Scholar
  27. Voorrips, R.E., M.C. Jongerius & H.J. Kanne, 1997. Mapping of two genes for resistance to clubroot (Plasmodiophora brassicae) in a population of doubled haploid lines of Brassica oleraceaby means of RFLP and AFLP markers. Theor Appl Genet 94: 75-82.CrossRefPubMedGoogle Scholar
  28. Williams, P.H., 1966. A system for the determination of races of Plasmodiophora brassicaethat infect cabbage and rutabaga. Phytopathology 56: 624-626.Google Scholar
  29. Wit, F., 1964. Inheritance of reaction to clubroot in turnips. Hortic Res 5: 47-49.Google Scholar

Copyright information

© Kluwer Academic Publishers 1998

Authors and Affiliations

  • Etsuo Matsumoto
    • 1
  • Chika Yasui
    • 2
  • Michio Ohi
    • 2
  • Motohisa Tsukada
    • 1
  1. 1.Department of Vegetable Breeding, MatsushiroNagano Vegetable & Ornamental Crops Experiment StationNaganoJapan
  2. 2.Faculty of Agriculture, Minami-minowaShinsyu UniversityKami-inaJapan

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