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Development of Novel Microsatellite Markers in Jatropha curcas and Evaluation of Their Cross-Species Transferability

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Abstract

Jatropha curcas has been a species of interest all over the world due to its potential as a renewable biodiesel crop. In the present paper, a set of 40 novel genomic microsatellites from Jatropha developed through cloning and sequencing are reported. The microsatellite markers were evaluated at intra-specific, inter-specific and inter-generic levels. All forty markers successfully amplified fragments from J. curcas and J. integerrima showing their potential in linkage mapping using inter-specific breeding populations involving these species. The average number of alleles within 12 core accessions of J. curcas was 3.083. Polymorphic information content ranged from 0.15 to 0.80 within J. curcas accessions and from 0 to 0.37 among different genera. There was no significant deviation from Hardy–Weinberg equilibrium or linkage disequilibrium. Ten of these microsatellites showed polymorphism within Emblica officinalis while thirteen were polymorphic within Phyllanthus niruri and Ricinus communis accessions. These new set of microsatellite markers are expected to be especially useful in future for linkage mapping in J. curcas and for varietal identification and genetic diversity studies in other genera mentioned above.

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Acknowledgments

The authors acknowledge the gracious funding provided by the Department of Biotechnology, Government of India. Pratima Sinha is thankful to the Ministry of New and Renewable Energy (MNRE), Government of India, for providing fellowship for this work. The authors are also thankful to Dr. R. K. Pachauri, Director General, TERI, for his kind support.

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. The Energy and Resources Institute, IHC Complex, Lodhi Road, New Delhi, 110 003, India

    Pratima Sinha, Md. Aminul Islam, Madan Singh Negi & Shashi Bhushan Tripathi

Authors
  1. Pratima Sinha
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  2. Md. Aminul Islam
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  3. Madan Singh Negi
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  4. Shashi Bhushan Tripathi
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Corresponding author

Correspondence to Shashi Bhushan Tripathi.

Electronic supplementary material

Below is the link to the electronic supplementary material. Supplementary Fig. 1. Dendrogram showing UPGMA clustering of 12 core accessions of Jatropha curcas. Bootstrap values over 50 were considered to be significant and are shown in the dendrogram.

Supplementary material 1 (TIFF 460 kb)

Supplementary material 2 (DOCX 31 kb)

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Sinha, P., Islam, M.A., Negi, M.S. et al. Development of Novel Microsatellite Markers in Jatropha curcas and Evaluation of Their Cross-Species Transferability. Proc. Natl. Acad. Sci., India, Sect. B Biol. Sci. 85, 1011–1016 (2015). https://doi.org/10.1007/s40011-015-0489-x

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  • DOI: https://doi.org/10.1007/s40011-015-0489-x

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