Abstract
Jatropha curcas L. (physic nut) is native to Central America and now naturalized widely in many tropical and subtropical areas. Microsatellite markers were isolated and characterized. Eleven out of 55 markers showed polymorphisms, and the allelic variation was investigated using 26 accessions of J. curcas collected from several provinces in Thailand. Each marker showed 2 to 5 alleles and the average polymorphic information content (PIC) was 0.49. Thirty four markers (62 %) were also successfully amplified in J. integerrima, J. gossypifolia and J. podagrica.
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Abbreviations
- AFLP:
-
amplified fragment length polymorphism
- ISSR:
-
inter simple sequence repeat
- PIC:
-
polymorphic information content
- RAPD:
-
randomly amplified polymorphic DNA
- SSR:
-
simple sequence repeats
References
Akkak, A., Scariot, V., Torello Marinoni, D., Boccacci, P., Beltramo, C., Botta, R.: Development and evaluation of microsatellite markers in Phoenix dactylifera L. and their transferability to other Phoenix species. — Biol. Plant. 53: 164–166, 2009.
Akritidis, P., Mylona, P.V., Tsaftaris, A.S., Polidoros, A.N.: Genetic diversity assessment in greek Medicago truncatula genotypes using microsatellite markers. — Biol. Plant. 53: 343–346, 2009.
Basha, S.D., Sujatha, M.: Genetic analysis of Jatropha species and interspecifichybrids of Jatropha curcas using nuclear and organelle specific markers. — Euphytica 168: 197–214, 2009.
Benbouza, H., Jacquemin, J.M., Baudoin, J.P., Mergeai, G.: Optimization of a reliable, fast, cheap and sensitive silver staining method to detect SSR markers in polyacrylamide gels. — Biotechnol. Agron. Soc. Environ. 10: 77–81, 2006.
Connell, J.P., Sujata, P., Iqbal, M.J., Huizinga, T., Ready, A,S.: A high through-put procedure for capturing microsatellite from complex plant genomes. — Plant. mol. Biol. Rep. 16: 341–349, 1998.
Doyle, J.J., Doyle, J.L.: Isolation of plant DNA from fresh tissue. — Focus 12: 13–15, 1990.
Ghosh, A., Chaudhary, D,R., Reddy, M,P., Rao, S,N., Chikara, J., Pandya, J.B.: Prospects for Jatropha methyl ester (biodiesel) in India. — Int. J. environ. Stud. 64: 659–674, 2007.
Hu, J.B., Zhou, X.Y., Li, J.W.: Development of novel chloroplast microsatellite markers for Cucumis from sequence database. — Biol. Plant. 53: 793–796, 2009.
Liu, K., Muse, S,V.: PowerMarker: an integrated analysis environment for genetic marker analysis. — Bioinformatics 21: 2128–2129, 2005.
Sudheer, P.D.V.N., Nirali, P., Reddy, M.P., Radhakrishnan, T.: Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP. — Mol. Biol. Rep. 36: 901–907, 2009a.
Sudheer, P.D.V.N., Singh, S., Mastan, S.G., Patel, J., Reddy, M.P.: Molecular characterization and identification of markers for toxic and non-toxic varieties of Jatropha curcas L. using RAPD, AFLP and SSR markers. — Mol. Biol. Rep. 36: 1357–1364, 2009b.
Acknowledgments
The authors gratefully acknowledge Drs. Kazuyoshi Hosaka and Paul G. Johnson for useful comments and improvement of the manuscript and Anna Saimaneerat, Panie Tongpumnuk and Isara Sooksathan for providing J. curcas. This research was financially supported by the Thai Research Fund and the Thai Commission on Higher Education.
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Phumichai, C., Phumichai, T., Kongsiri, N. et al. Isolation of 55 microsatellite markers for Jatropha curcas and its closely related species. Biol Plant 55, 387–390 (2011). https://doi.org/10.1007/s10535-011-0061-3
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DOI: https://doi.org/10.1007/s10535-011-0061-3