Skip to main content
SpringerLink
Log in

Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP

Genetic divergence and phylogenic analysis of genus Jatropha

  • Published:
Molecular Biology Reports Aims and scope Submit manuscript

Abstract

Genus Jatropha with 172 species having significant economic importance belongs to the family Euphorbiaceae. There are no reports on molecular characterization and phylogenetic relationship among the species of Jatropha. Hence, the present study was undertaken to assess the extent of genetic variability that exist and also to establish phylogenetic relationship among Jatropha curcas, J. glandulifera, J. gossypifolia, J. integerrima, J. multifida, J. podagrica and J. tanjorensis using RAPD and AFLP. The percentage of loci that are polymorphic among the species studied was found to be 97.74% by RAPD and 97.25% by AFLP. The mean percentage of polymorphism (PP) was found to be 68.48 by RAPD and 71.33 by AFLP. The phylogram generated with RAPD and AFLP data showed maximum similarity. With the generated data maximum relatedness was found between J. curcas and J. integerrima this may be the reason for the success of inter hybrid crosses between these two species. Neither RAPD nor AFLP data generated in this study supports the view of J. tanjorensis, a natural interspecific hybrid between J. curcas and J. gossypifolia. The present study concludes that both RAPD and AFLP techniques are comparable in divergence studies of Jatropha species. The markers generated by RAPD and AFLP can be employed efficiently for interspecific hybrids identification, marker assisted selection and genetic resource management.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  1. Fairless D (2007) Biofuel: the little shrub that could - maybe. Nature 449:652–655

    Article  PubMed  Google Scholar 

  2. Sunita K, Kochar VK, Singh SP, Katiyar RS, Pushpangadan P (2005) Differential rooting and sprouting behaviour of two Jatropha species and associated physiological and biochemical changes. Curr Sci 89:936–939

    Google Scholar 

  3. Olapeju OA, Kayode A, Olusegun E, James BG (2007) Antibacterial diterpenoids from Jatropha podagrica Hook. Phytochemistry 68:2420–2425

    Article  Google Scholar 

  4. Prabakaran AJ, Sujatha M (1999) Jatropha tanjorensis Ellis & Saroja, a natural interspecific hybrid occurring in Tamil Nadu, India. Gene Res Crop Evol 46:213–218

    Article  Google Scholar 

  5. Yotam L, Yaniv S, Haim B, Menachem S, Emile H (2000) Rare Jatropha multifida intoxication in two children. J Emerg Med 19:173–175

    Article  Google Scholar 

  6. Taofeeq O, Ganiyu OA, Tesleem AO, Godwin OA, Mutiyat AO (2005) Mechanism of action of Jatropha gossypifolia stem latex as a haemostatic agent. Eur J Gen Med 2(4):140–143

    Google Scholar 

  7. Ramchandani M, Jolly CI (1988) Pharmacognostical and phytochemical studies on Phyllanthus fraternus webster and Jatropha glandulifera ROXB. Indian J Pharm Sci 50:276–277

    CAS  Google Scholar 

  8. Banerji R, Chowdhury AR, Misra G, Sudarsanam G, Verma SC, Srivastava GS (1985) J. curcas seed oil for energy. Biomass 8:277–282

    Article  CAS  Google Scholar 

  9. Gubitz GM, Mittelbach M, Trabi M (1999) Exploitation of the tropical oil seed plant Jatropha curcas L. Bioresour Technol 67:73–82

    Article  CAS  Google Scholar 

  10. Mandpe S, Kadlaskar S, Degen W, Keppeler S (2005) On road testing of advanced common Rail diesel vehicles with biodiesel from the Jatropha Curcas plant, Society of Automotive Engineers. Inc 26:356–364

    Google Scholar 

  11. Martin G, Mayeux A (1984) Réflexions sur les cultures oléagineuses énergétiques. II. - Le Pourghère (Jatropha curcas L.): UN carburant possible. Oléagineux 39:283–287

    Google Scholar 

  12. Staubmann R, Ncube I, Gubitz GM, Steiner W, Read JS (1999) Esterase and lipase activity in Jatropha curcas L. J Biotechnol 75:117–126

    Article  PubMed  CAS  Google Scholar 

  13. Takeda Y (1982) Development study on Jatropha curcas (Sabudum) oil as a substitute for diesel engine oil in Thailand. J Agric Assoc China 120:1–8

    Google Scholar 

  14. Ginwal HS, Rawat PS, Srivastava RL (2004) Seed source variation in growth performance and oil yield of Jatropha curcas Linn. in central India. Silve Senetica 53:186–192

    Google Scholar 

  15. Doyle JJ, Doyle JL (1990) Isolation of fresh DNA from fresh tissue. Focus 12:3–15

    Google Scholar 

  16. Williams JG, Kubelik AR, Livak J, Rafalski J, Tingey SV (1990) DNA polymorphism amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Res 18:6531–6535

    Article  PubMed  CAS  Google Scholar 

  17. Vos P, Hogers R, Bleeker M, Reijans M, de Lee TV, Hornes M, Frijters A, Pot J, Peleman J, Kuiper M, Zabeau M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414

    Article  PubMed  CAS  Google Scholar 

  18. Jaccard P (1908) Nouvelles recherchesur la distribution florale. Bull Soc Vaud Sci Natl 44:223–270.

    Google Scholar 

  19. Felsenstein J (1985) Confidence limits on phylogenies: an approach using the bootstrap. Evolution 39:783–791

    Article  Google Scholar 

  20. Francisco-Ortega J, Crawford DJ, Santos-Guerra A, Cravalho JA (1996) Isozyme differentiation in the endemic genus Argyranthemum (Asteraceae, Anthemideae) in the Macaronesian islands. Plant Syst Evol 202:137–152

    Article  CAS  Google Scholar 

  21. Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In Brown HD et al (eds) Plant population genetics, breeding and genetic resources. Sinaue Sunderland, MA, USA, pp 43–63

  22. Crawford DJ, Haines DW, Cosmer MB, Wiens D, Lopez P (1994) Lactoris fernandeziana on the Juan Fernandez Islands allozyme uniformity and field obsevarvations. Conserv Biol 8:277–280

    Article  Google Scholar 

  23. Essilman EJ, Crawford DJ, Brauner S, Stuessy TF, Anderson GJ, Silva OM (1997) RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae). Am J Bot 4:591–596

    Google Scholar 

  24. Francisco-Ortega J, Crawford DJ, Santos-Guerra A, Cravalho JA (1996) Isozyme differentiation in the endemic genus Argyranthemum (Asteraceae, Anthemideae) in the Macaronesian islands. Plant Syst Evol 202:137–152

    Article  CAS  Google Scholar 

  25. Lesica P, Leary RF, Allendort FR, Bilderbecl DE (1998) Lac of genetic diversity within and among populations of an endangered plant, Hawellia aquatilis. Conserv Biol 2:275–282

    Article  Google Scholar 

  26. Lowrey TK, Crawroford DJ (1985) Allozyme divergence and evolution in Tertamolopium (Compositae; Astereae) on the Hawaiian Island. Syst Bot 10:64–72

    Article  Google Scholar 

  27. Soltis PS, Soltis DE, Tucker TL, Lang FA (1992) Allozyme variability is absent in the narrow endemic Bensoniella oregona (Saifragacear). Conserv Biol 6:131–134

    Article  Google Scholar 

  28. Adams RP, Demeke T (1993) Systematic relationships in Juniperus based on random amplified polymorphic DNAs (RAPDs). Taxon 42:553–571

    Article  Google Scholar 

  29. Campos LP, Raelson JV, Grant WF (1994) Genome relation ships among lotus species based on random amplified polymorphic DNA (RADP). Theor Appl Genet 88:417–422

    Article  CAS  Google Scholar 

  30. Catalan P, Shi Y, Armstrong L, Draper J, Stace CA (1995) Molecular phylogeny of the grass genus Brachypodium P. beauv. based on RFLP and RAPD analysis. Bot J Linn Soc 117:263–280

    Article  Google Scholar 

  31. Essilman EJ, Crawford DJ, Brauner S, Stuessy TF, Anderson GJ, Silva OM (1997) RAPD marker diversity within and divergence among species of Dendroseris (Asteraceae: Lactuceae). Am J Bot 4:591–596

    Google Scholar 

  32. Ranade SA, Kumar A, Goswami M, Farooqui N, Sane ΡV (1997) Genome analysis of Amaranths: determination of inter and intra-species variations. J Biosci 22:457–464

    Article  Google Scholar 

  33. Spooner DM, Ugarte L, Scroch PW (1997) Species boundaries and interrelationships of two closely related sympatric diploid wild potato species, Solanum astleyl and Solanum bollviense, based on RAPDs. Theor Appl Genet 96:764–771

    Article  Google Scholar 

  34. Brucci G, Menozzi P (1993) Segregation analysis of random amplified polymorphic DNA (RAPD) markers in Picea abies Karst. Mol Ecol 2:227–232

    Article  Google Scholar 

  35. Brunell MS, Whitkus R (1997) RAPD marker veration in Eriastrum densifolium (polemoniaceae), implications for sub specific delimitation and conservation. Syst Bot 22:543–553

    Article  Google Scholar 

  36. Volis S, Yakubov B, Shulagina I, Ward D, Zur V, Mendlinger S (2001) Tests for adaptive RAPD variation in population genetic structure of wild barley, Hordeum spontaneum Koch. Biol J Linn Soc 74:289–303

    Article  Google Scholar 

  37. Brunell MS, Whitkus R (1997) RAPD marker veration in Eriastrum densifolium (Polemoniaceae), implications for sub specific delimitation and conservation. Syst Bot 22:543–553

    Article  Google Scholar 

  38. Hadrys H, Balick M, Schierwater B (1992) Applications of random amplified polymorphic DNA(RAPD) in molecular ecology. Mol Ecol 1:55–263

    Article  PubMed  CAS  Google Scholar 

  39. Basha SD, Sujatha M (2007) Inter and intra-population variability of Jatropha curcas (L.) characterized by RAPD and ISSR markers and development of population-specific SCAR markers. Euphytica 156:375–386

    Article  CAS  Google Scholar 

  40. Ganesh RS, Parthiban KT, Senthil KR, Thiruvengadam V, Paramathma M (2008) Genetic diversity among Jatropha species as reveled by RAPD markers. Genet Resour Crop Evol. doi:10.1007/s10722-007-9285-7

    Google Scholar 

  41. Sujatha M, Prabakaran AJ (2003) New ornamental Jatropha hybrids through interspecific hybridization. Genet Resour Crop Evol 50:75–82

    Article  CAS  Google Scholar 

  42. Sujatha M, Makkar HPS, Becker K (2005) Shoot bud proliferation from axillary nodes and leaf sections of non-toxic Jatropha curcas L. Plant Growth Regul 47:83–90

    Article  CAS  Google Scholar 

  43. Soontornchinaksang P, Jenjittikul T (2003) Karyology of Jatropha (Euphorbiaceae). Thai For Bull (Bot) 31:105–112

    Google Scholar 

  44. Rupert EA, Dehgan B, Webster GL (1970) Experimental studies of relationships in the genus Jatropha. I. J. curcas X J. integerrima. Bull Torrey Bot Club 99:321–325

    Article  Google Scholar 

  45. Bussel JD, Michelle W, Jennifer AC (2005) Arbitrarily amplified DNA markers as characters for phylogenetic inference. Perspect Plant Ecol Evol Syst 7:3–26

    Article  Google Scholar 

Download references

Acknowledgements

We are grateful to Dr. P. K. Ghosh, Director CSMCRI for encouragement and Dimyler Chryslar, Germany and CSIR New Delhi for financial support.

Author information

Authors and Affiliations

  1. Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute, Bhavnagar, Gujarat, 364002, India

    D. V. N. Sudheer Pamidiamarri, Nirali Pandya & Muppala P. Reddy

  2. National Research Center for Groundnut, Junagadh, 362 001, Gujarat, India

    T. Radhakrishnan

Authors
  1. D. V. N. Sudheer Pamidiamarri
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Nirali Pandya
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Muppala P. Reddy
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Radhakrishnan
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Muppala P. Reddy.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Sudheer Pamidiamarri, D.V.N., Pandya, N., Reddy, M.P. et al. Comparative study of interspecific genetic divergence and phylogenic analysis of genus Jatropha by RAPD and AFLP. Mol Biol Rep 36, 901–907 (2009). https://doi.org/10.1007/s11033-008-9261-0

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s11033-008-9261-0

Keywords

Search

Navigation