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Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites

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Abstract

Jatropha curcas L. belongs to family Euphorbiaceae, native to South America attained significant importance for its seed oil which can be converted to biodiesel, a renewable energy source alternative to conventional petrodiesel. Very few attempts were made to isolate novel microsatellite markers and assessment of the extent of genetic equilibrium and diversity that exists in J. curcas. Therefore, the present investigation was undertaken to isolate the novel microsatellites and access genetic equilibrium, diversity that exists among 44 diverse germplasm collected from distinct geographical areas in India using isolated microsatellites. The overall efficiency of the enrichment of microsatellite by dual probe in the present study found to be 54% and among the sequences obtained the percentage of sequences having suitable flanking regions for the primer designing was found to be 89.58%. The mean co-efficient of genetic similarity (CGS) was found to be 0.97. The overall diversity obtained by microsatellites was found to be low in comparison with the diversity reported by multilocus markers systems observed in earlier studies; however, the good allele polymorphism was observed. The overall dendrogram of microsatellite analysis resulted in random clustering of germplasm and not in accordance to geographical area of collection. The present study, diversity analysis using microsatellite markers concludes the low genetic diversity and genetic disequlibrium of J. curcas in India and will provide pavement for further intra-population studies on narrow geographical areas to understand the population genetic structure, phylogeography and molecular ecological studies. The germplasm characterized, and the microsatellite markers isolated and characterized in the present study can be employed efficiently in breeding programs for genetic improvement of the species through marker assisted selection and QTL analysis, for further genetic resource management and help in making the J. curcas as potential crop with superior agronomical traits.

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Acknowledgments

The authors wish to thank Council for Scientific and Industrial Research (CSIR), New Delhi, India for financial support and for the Research Associate fellowship.

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Author notes

  1. Pamidimarri D. V. N. Sudheer

    Present address: Shree M. & N. Virani Science College, Saurashtra University, Rajkot, Gujarat, 360005, India

  2. Muppala P. Reddy

    Present address: Plant Stress Genomics and Technology Center, King Abdullah University of Science and Technology, Thuwal, 23955-6900, Kingdom of Saudi Arabia

Authors and Affiliations

  1. Discipline of Wasteland Research, Central Salt and Marine Chemicals Research Institute (CSIR), Bhavnagar, Gujarat, 364002, India

    Pamidimarri D. V. N. Sudheer, Hifzur Rahman, Shaik G. Mastan & Muppala P. Reddy

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  1. Pamidimarri D. V. N. Sudheer
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  2. Hifzur Rahman
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  3. Shaik G. Mastan
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  4. Muppala P. Reddy
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Correspondence to Muppala P. Reddy.

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Sudheer, P.D.V.N., Rahman, H., Mastan, S.G. et al. Isolation of novel microsatellites using FIASCO by dual probe enrichment from Jatropha curcas L. and study on genetic equilibrium and diversity of Indian population revealed by isolated microsatellites. Mol Biol Rep 37, 3785–3793 (2010). https://doi.org/10.1007/s11033-010-0033-2

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