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Preparation and in vivo evaluation of photo-cured O-carboxymethyl chitosan micro-particle for controlled drug delivery

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Abstract

Medical advances have prompted the research and development of biomaterials. Especially, chitosan has emerged as new generation of biomaterials because it beneficial properties as a natural polymer, such as less toxic, biodegradable, and biocompatible than synthetic ones. The prepared azidophenyl O-carboxymethyl chitosan (Az-O-CMC) was characterized by FTIR, 1H NMR and particle-size analyzer. The hardening rate and cross-linking maintenance of the Az-O-CMC were investigated with its irradiation time and concentration. A photo-mask was used for observation of photoimmobilization. 3T3-L1 cells were cultured to determine effect of cytotoxicity. Protein release test was conducted to evaluate the immobilizing effect. In addition, animal test was investigated for wound healing application. In this study, the results indicate that immobilization of protein with Az-O-CMC will be useful for medical application.

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Authors and Affiliations

  1. Department of Systems Biotechnology and Bio-Environmental Technology (BET) Research Institute, Chung-Ang University, Gyeonggi, 456-756, Korea

    Yun Heo, Shin-Hye Park, Si-Yoong Seo & Tae-Il Son

  2. Korea Research Institute of Standards and Science, Yuseong, Daejeon, 305-340, Korea

    Ju-Young Yun

  3. Nano Medical Engineering Laboratory, RIKEN Advanced Science Institute, 2-1 Hirosawa, Wako, Saitama, 351-0198, Japan

    Yoshihiro Ito

Authors
  1. Yun Heo
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  2. Shin-Hye Park
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  3. Si-Yoong Seo
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  4. Ju-Young Yun
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  5. Yoshihiro Ito
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  6. Tae-Il Son
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Correspondence to Tae-Il Son.

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Heo, Y., Park, SH., Seo, SY. et al. Preparation and in vivo evaluation of photo-cured O-carboxymethyl chitosan micro-particle for controlled drug delivery. Macromol. Res. 22, 541–548 (2014). https://doi.org/10.1007/s13233-014-2079-9

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  • DOI: https://doi.org/10.1007/s13233-014-2079-9

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