Abstract
In this study, we have successfully synthesized magnetic nanoparticles (MNPs), functionalised them by silanization and used them for the covalent immobilization of a recombinant small laccase (rSLAC) from Streptomyces coelicolor. The immobilized recombinant laccase (MNP-rSLAC) was subsequently used for the treatment of phenol, 4-chlorophenol (4-CP) and 4-fluorophenol (4-FP). The enzyme completely degraded 80 µg/mL of the selected phenolic compounds within 2 h in the presence of a natural mediator, acetosyringone. The MNP-rSLAC retained > 73% of initial activity (2,6-dimethoxyphenol as substrate) after 10 catalytic cycles and could be easily recovered from the reaction mixture by the application of magnetic field. Furthermore, immobilised rSLAC exhibited better storage stability than its free counterpart. The Michaelis constant (Km) value for the immobilised rSLAC was higher than free rSLAC, however the maximum velocity (Vmax) of the immobilised SLAC was similar to that of the free rSLAC. Growth inhibition studies using Escherichia coli showed that rSLAC-mediated treatment of phenolic compounds reduced the toxicity of phenol, 4-CP and 4-FP by 90, 60 and 55%, respectively. Interestingly, the presence of selected metal ions (Co2+, Cu2+, Mn2+) greatly enhanced the catalytic activity of rSLAC and MNP-rSLAC. This study indicates that immobilized small laccase (MNP-rSLAC) has potential for treating wastewater contaminated with phenolic compounds.
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The authors acknowledge the National Research Foundation (South Africa) for financial support (Grant 105889 and 112099). Any opinion, findings and conclusions or recommendations expressed in this material are those of the authors and therefore the NRF does not accept any liability in regard thereto. Financial support from the Council of Scientific and Industrial Research (CSIR) for student (Deepti Yadav) scholarship is gratefully acknowledged.
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The authors acknowledge the National Research Foundation (South Africa) for financial support (Grant 105889 and 112099). Any opinion, findings and conclusions or recommendations expressed in this material are those of the authors and therefore the NRF does not accept any liability in regard thereto. Financial support from the Council of Scientific and Industrial Research (CSIR) for student (Deepti Yadav) scholarship, is gratefully acknowledged.
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DY—Investigation, Methodology, Writing—original draft; BR—Investigation, Methodology; NM—Conceptualisation, Supervision; MLR-H—Conceptualisation, Supervision, Writing—review and editing; TK—Conceptualisation, Supervision, Writing-review and editing, Funding acquisition.
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Yadav, D., Ranjan, B., Mchunu, N. et al. Enzymatic treatment of phenolic pollutants by a small laccase immobilized on APTES-functionalised magnetic nanoparticles. 3 Biotech 11, 302 (2021). https://doi.org/10.1007/s13205-021-02854-0
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DOI: https://doi.org/10.1007/s13205-021-02854-0