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Immobilization of laccase by Cu2+ chelate affinity interaction on surface-modified magnetic silica particles and its use for the removal of 2,4-dichlorophenol

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Abstract

Magnetic Cu2+-chelated silica particles that employ polyacrylamide as a metal-chelating ligand were developed and used to immobilize laccase by coordination. The particles were characterized by scanning electron microscope and Fourier transform infrared spectroscopy. The preparation, the enzymatic properties of the immobilized laccase, and its catalytic capacity for 2,4-dichlorophenol degradation were systemically evaluated. The results showed that immobilized laccase exhibited maximum enzyme activity when it was immobilized for 1 h at a pH of 4.0 and a temperature of 5 °C. The optimum laccase dose was 20 mg/g of carrier. In comparison to free laccase, the immobilized laccase had better acid adaptability and thermal stability. Higher activity was observed for immobilized laccase at a pH range of 2.0 to 3.5 and temperatures from 25 to 40 °C. The immobilized laccase that was prepared for this work exhibited a good catalytic capacity for removing 2,4-dichlorophnol from aqueous solutions.

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Acknowledgments

This work was supported by the International Science & Technology Cooperation Program of China (grant no. 2013DFR90290), National Natural Science Foudation of China (grant no. 21177013, 50708007, and 40961029) and the fundamental Research Funds for the Central Universities.

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Authors and Affiliations

  1. The Key Laboratory of Water and Sediment Sciences, Ministry of Education, School of Environment, Beijing Normal University, Beijing, 100875, People’s Republic of China

    Ying Wang & Ran Wang

  2. Department of Chemical Engineering, Beijing University of Chemical Technology, Beijing, 100029, People’s Republic of China

    Xiaochun Chen & Furong He

  3. Ministry of Transport, Transport Planning and Research Institute, Beijing, 100028, China

    Jie Liu

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  1. Ying Wang
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  2. Xiaochun Chen
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  3. Jie Liu
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  4. Furong He
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  5. Ran Wang
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Correspondence to Ying Wang.

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Responsible editor: Vinod Kumar Gupta

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Wang, Y., Chen, X., Liu, J. et al. Immobilization of laccase by Cu2+ chelate affinity interaction on surface-modified magnetic silica particles and its use for the removal of 2,4-dichlorophenol. Environ Sci Pollut Res 20, 6222–6231 (2013). https://doi.org/10.1007/s11356-013-1661-6

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  • DOI: https://doi.org/10.1007/s11356-013-1661-6

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