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In Vitro Propagation and Synthetic Seeds Production: An Efficient Methods for Stevia rebaudiana Bertoni

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Abstract

Germplasm can be effectively stored in the form of synthetic seeds. Shoot tips obtained from in vitro shoot cultures of Stevia rebaudiana Bertoni were encapsulated in 4 % calcium alginate. The present work discussed the role of components of culture medium on morphogenic response of Stevia rebaudiana Bertoni encapsulated buds to various MS strengths and sugar alcohol (Mannitol or sorbitol) in different concentrations for long term storage. Germination ability of the synthetic seeds was investigated. Shoots were regenerated from nodal explants of Stevia through axillary shoot proliferation. The induction of multiple shoots from nodal segments was the highest in MS medium supplemented with 1.0 mg l−1 BA. Maximum shoot formation was obtained with fructose at 20 and 40 g l−1 fructose, while with fructose at 20 g l−1 gave the highest leaves number/explants). The longest shoot length obtained with sucrose and fructose than other sugar. Different media type (NN and WPM) were suitable for best shoot number of Stevia, leaf number and shoot length than other media. Growth of shoot was increased and observed in capsules stored for 5 weeks on MS than other MS strengths. The growth of capsules dependent on mineral concentration and storage time. The most suitable conversions of capsules was using 0.05 M mannitol after 6 weeks from storage of synthetic seeds of Stevia. For rooting, when Stevia shoots cultured on MS medium supplemented with IAA, at 0.2 mg l−1 resulted in the maximum number of roots/explant while, IBA at 1.0 and 2.0 mg l−1 resulted in longest root/plant and gave the same length of root.

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  1. Department of Plant Biotechnology, Genetic Engineering and Biotechnology Research Institute (GEBRI), Menofyia University, Sadat City, Egypt

    Ahmed Abbas Nower

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  1. Ahmed Abbas Nower
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Nower, A.A. In Vitro Propagation and Synthetic Seeds Production: An Efficient Methods for Stevia rebaudiana Bertoni. Sugar Tech 16, 100–108 (2014). https://doi.org/10.1007/s12355-013-0228-7

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