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Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi baker

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Abstract

Somatic embryos of Asparagus cooperi were encapsulated as single embryos approximately 4–6 mm in diameter to produce individual synthetic seeds. The frequency of conversion of artificial seeds to plants was 34%. This frequency was affected by the concentration of calcium chloride, the commercial sources of sodium alginate, and the nutrient medium. The conversion frequency of artificial seeds to seedling plants was 8.3% after storage for 90 days at 2°C.

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Abbreviations

NAA:

α-Naphthaleneacetic acid

ABA:

Abscisic acid

GA3 :

Gibberellic acid

Kn:

6-Furfurylaminopurine

MS:

Murashige and Skoog (1962) medium

SH:

Schenk and Hildebrandt (1972) medium

WM:

White (1963) medium

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Authors and Affiliations

  1. Centre of Advanced Study, Department of Botany, University of Calcutta, 35, Ballygunge Circular Road, 700019, Calcutta, India

    Biswajit Ghosh & Sumitra Sen

Authors
  1. Biswajit Ghosh
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  2. Sumitra Sen
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Additional information

Communicated by J. J. Finer

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Ghosh, B., Sen, S. Plant regeneration from alginate encapsulated somatic embryos of Asparagus cooperi baker. Plant Cell Reports 13, 381–385 (1994). https://doi.org/10.1007/BF00234142

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  • DOI: https://doi.org/10.1007/BF00234142

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