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Reproductive sink enhanced drought induced senescence in wheat fertile line is associated with loss of antioxidant competence compared to its CMS line

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Abstract

Reproductive sinks regulate monocarpic senescence in wheat as desinking delayed flag leaf senescence under irrigated condition. In this study, wheat cv. HW 2041 and its isonuclear male sterile line (CMS) were subjected to post-anthesis water deficit stress to understand the association between sink strength, senescence and drought response in relation to oxidative stress and antioxidant defense at cellular and sub-cellular level. CMS plants maintained better water relations and exhibited delayed onset and progression of flag leaf senescence in terms of green leaf area, chlorophyll and protein content than fertile plants under water deficit stress (WDS). Delayed senescence in CMS plants under water deficit stress was associated with less reactive oxygen species generation, lower damage to membranes and better antioxidant defense both in terms of antioxidant enzyme activities and metabolite content compared to fertile plants. Expression of some senescence associated genes (SAGs) such as WRKY transcription factor (WRKY53), glutamine synthetase1 (GS1), wheat cysteine protease (WCP2) and wheat serine protease (WSP) was lower while catalse 2 (CAT2) transcript levels were higher in the CMS plants compared to HW2041 during senescence under water deficit stress. Antioxidant defense in chloroplasts was better in CMS line under water deficit stress compared to HW2041. This is the first report showing that reproductive sink enhanced drought induced senescence in flag leaf of wheat fertile line is associated with higher oxidative stress and damage and loss of antioxidant competence compared to its sterile line under water deficit stress. Higher expression of some SAGs and decline in superoxide dismutase and ascorbate peroxidase activity in the chloroplasts also contributed to the accelerated senescence in fertile line compared to its CMS line under WDS.

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Abbreviations

AsA:

Ascorbic acid reduced

APX:

Ascorbate peroxidase

CAT:

Catalase

DHA:

Ascorbic acid oxidized

DAA:

Days after anthesis

GS1:

Glutamine synthetase

GSH:

Glutathione reduced

GSSG:

Glutathione oxidized

ROS:

Reactive oxygen species

SOD:

Superoxide dismutase

SAGs:

Senescence associated genes

WCP:

Wheat cysteine protease

WSP:

Wheat serine protease

WDS:

Water deficit stress

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Acknowledgements

Dr. (Mrs.) R. Khanna-Chopra was awarded Emeritus Scientist Scheme by Council of Scientific and Industrial Research India which supported the present research. VKS thanks Council of Scientific and Industrial Research India for Research Associate fellowship.

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  1. Renu Khanna-Chopra

    Present address: Africa Rice Center (AfricaRice), C/O IITA, PMB 5320, Oyo Road, Ibadan, Nigeria

  2. Renu Khanna-Chopra is INSA Senior Scientist.

Authors and Affiliations

  1. Stress Physiology Lab, Water Technology Centre, Indian Agricultural Research Institute, New Delhi, 110012, India

    Vimal Kumar Semwal & Renu Khanna-Chopra

  2. Africa Rice Center (AfricaRice), C/O IITA, PMB 5320, Oyo Road, Ibadan, Nigeria

    Vimal Kumar Semwal

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Supplementary Fig. 1.

Effect of water deficit stress on the expression of senescence associated genes WRKY53, GS1, WCP2, WSP and CAT2 genes in flag leaf of wheat cv. HW2041 and its CMS line during monocarpic senescence under water deficit stress (TIFF 21636 kb)

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Semwal, V.K., Khanna-Chopra, R. Reproductive sink enhanced drought induced senescence in wheat fertile line is associated with loss of antioxidant competence compared to its CMS line. Physiol Mol Biol Plants 24, 591–604 (2018). https://doi.org/10.1007/s12298-018-0549-9

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