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Growth and consumption of l-malic acid in wine-like medium by acclimated and non-acclimated cultures of Patagonian Oenococcus oeni strains

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Abstract

Five Oenococcus oeni strains, selected from spontaneous malolactic fermentation (MLF) of Patagonic Pinot noir wine, were assessed for their use as MLF starter cultures. After the individual evaluation of tolerance to some stress conditions, usually found in wine (pH, ethanol, SO2, and lysozyme), the behavior of the strains was analyzed in MLO broth with 14 % ethanol and pH 3.5 in order to test for the synergistic effect of high ethanol level and low pH and, finally, in a wine-like medium. Although the five strains were able to grow in MLO broth under low pH and/or high ethanol, they must be acclimated to grow in a wine-like medium. Additionally, glycosidase and tannase activities were evaluated, showing differences among the strains. The potential of the strains to ferment citrate was tested and two of the five strains showed the ability to metabolize this substrate. We did not detect the presence of genes encoding histidine, tyrosine descarboxylase, and putrescine carbamoyltransferase. All the strains tested exhibited good growth capacity and ability to consume l-malic acid in a wine-like medium after cell acclimation, and each of them showed a particular enzyme profile, which might confer different organoleptic properties to the wine.

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Acknowledgments

This work was funded by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicaciones Biotecnológicas), Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA), and ANPCyT (PICTO UNQ 2006 N° 36474, PICT SU 2012 N° 2804, PICT 2013 N° 1481). BMBF and NB are fellows of Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). LS is a member of the Research Career of CIC-BA; AH and ET are members of the Research Career of CONICET.

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Authors and Affiliations

  1. Laboratorio de Microbiología Molecular, Instituto de Microbiología Básica y Aplicada (IMBA), Departamento de Ciencia y Tecnología, Universidad Nacional de Quilmes (UNQ), Roque Sáenz Peña N° 352, (B1876BXD), Bernal, Buenos Aires, Argentina

    Bárbara Mercedes Bravo-Ferrada, Axel Hollmann, Natalia Brizuela, Danay Valdés La Hens, Elizabeth Tymczyszyn & Liliana Semorile

  2. Laboratorio de Biointerfaces y Sistemas Biomiméticos – Universidad Nacional de Santiago del Estero (UNSE) y Centro de Investigaciones y Transferencia de Santiago del Estero (CITSE) – CONICET y UNSE, Santiago del Estero, Argentina

    Axel Hollmann

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  1. Bárbara Mercedes Bravo-Ferrada
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  2. Axel Hollmann
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  3. Natalia Brizuela
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  5. Elizabeth Tymczyszyn
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Correspondence to Bárbara Mercedes Bravo-Ferrada.

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ESM 1: Supplementary 1

Clustering of O. oeni isolates from Patagonian Pinot noir wine. The dendrogram was obtained by UPGMA analysis of Coc, M13, and 1254 RAPD-PCR patterns. The reference strain is O. oeni ATCC 27310 = 27310. (JPG 1014 kb)

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Bravo-Ferrada, B.M., Hollmann, A., Brizuela, N. et al. Growth and consumption of l-malic acid in wine-like medium by acclimated and non-acclimated cultures of Patagonian Oenococcus oeni strains. Folia Microbiol 61, 365–373 (2016). https://doi.org/10.1007/s12223-016-0446-y

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