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Bacterial biodiversity and dynamics during malolactic fermentation of Tempranillo wines as determined by a culture-independent method (PCR-DGGE)

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Abstract

The bacterial population during malolactic fermentation of Tempranillo wine was studied using the polymerase chain reaction-denaturing gradient gel electrophoresis, a culture-independent method successfully used for identification and monitoring of bacterial population in different habitats included food fermentations. The results showed that Oenococcus oeni was the predominant species in the malolactic fermentation of Tempranillo wines, although the presence of Gluconobacter oxydans, Asaia siamensis, Serratia sp., and Enterobacter sp. was also observed. These results were partly coincidental with those obtained from a culture-dependent method, using a selective medium. Therefore, it may be concluded that for a more complete knowledge of the bacterial community present during malolactic fermentation of Tempranillo wine, an approach that combines a culture-independent method and a culture-dependent method would be advisable.

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Acknowledgments

The authors wish to thank the Ministry of Education and Science (INIA-MEC) for its financial support (project RM 2006-00011-C02-02). P. Ruiz is supported by a grant from the Council of Communities of Castilla-La Mancha (JCCM).

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Authors and Affiliations

  1. Departamento de Química Analítica y Tecnología de Alimentos, Facultad de Ciencias del Medio Ambiente, Universidad de Castilla—La Mancha, Avda. Carlos III s/n, 45071, Toledo, Spain

    Patricia Ruiz, Susana Seseña & María Llanos Palop

  2. Instituto de la Vid y del Vino de Castilla—La Mancha, Crta. Toledo-Albacete s/n, Tomelloso, 13700, Ciudad Real, Spain

    Pedro Miguel Izquierdo

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  1. Patricia Ruiz
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  2. Susana Seseña
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  3. Pedro Miguel Izquierdo
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  4. María Llanos Palop
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Correspondence to María Llanos Palop.

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Ruiz, P., Seseña, S., Izquierdo, P.M. et al. Bacterial biodiversity and dynamics during malolactic fermentation of Tempranillo wines as determined by a culture-independent method (PCR-DGGE). Appl Microbiol Biotechnol 86, 1555–1562 (2010). https://doi.org/10.1007/s00253-010-2492-8

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  • DOI: https://doi.org/10.1007/s00253-010-2492-8

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