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Patagonian red wines: selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation

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Abstract

The aim of this study was to evaluate fifty-three Lactobacillus plantarum isolates obtained from a Patagonian red wine, molecularly identified and typified using RAPD analysis, in order to select starter cultures for malolactic fermentation (MLF). The results obtained suggest a considerable genetic diversity, taking into account that all L. plantarum isolates were obtained from one cellar and one vintage. Based on the capacity to tolerate a concentration of 14 % ethanol in MRS broth for 2 days, eight isolates were selected for the subsequent analysis. The incidence of various wine stress factors (ethanol, acid pH, lysozyme and sulfur dioxide) on isolates growth was studied. Besides, glucosidase and tannase activities were evaluated, and the presence of genes involved in the synthesis of biogenic amines was examined by PCR. A previously characterized indigenous Oenococcus oeni strain was included with comparative purposes. Differences in technologically relevant characteristics were observed among the eight L. plantarum selected isolates, revealing an isolate-dependent behavior. Detectable glucosidase and tannase activities were found in all isolates. The presence of genes encoding histidine and tyrosine descarboxylases and putrescine carbamoyltransferase was not detected. The ability of L. plantarum isolates to grow and consume l-malic acid in simulated laboratory-scale vinifications revealed that two of them could be considered as possible MLF starter cultures for Patagonian red wines. These isolates will be subjected to further analysis, for a final winery technological characterization.

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Acknowledgments

This work was supported by grants from Universidad Nacional de Quilmes (Programa Microbiología Molecular Básica y Aplicaciones Biotecnológicas and ANPCyT—PICTO UNQ 2006 Nº 36474). Bravo Ferrada BM was recipient of a fellowship from Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET). Dr. L. Semorile is member of the Research Career of Comisión de Investigaciones Científicas de la Provincia de Buenos Aires (CIC-PBA)

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Authors and Affiliations

  1. Laboratorio de Microbiología Molecular, Departamento de Ciencia y Tecnología, Instituto de Microbiología Básica y Aplicada (IMBA), Universidad Nacional de Quilmes, Roque Sáenz Peña 352, B1876BXD, Bernal, Buenos Aires, Argentina

    Bárbara Mercedes Bravo-Ferrada, Axel Hollmann, Lucrecia Delfederico, Danay Valdés La Hens & Liliana Semorile

  2. Facultad de Ciencia y Tecnología de los Alimentos, Universidad Nacional del Comahue, 25 de Mayo y Reconquista, 8336, Villa Regina, Río Negro, Argentina

    Adriana Caballero

  3. IDEPA CONICET-Universidad Nacional del Comahue, 8300, Buenos Aires 1400, Neuquén, Argentina

    Adriana Caballero

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  1. Bárbara Mercedes Bravo-Ferrada
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  2. Axel Hollmann
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  3. Lucrecia Delfederico
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  4. Danay Valdés La Hens
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  5. Adriana Caballero
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  6. Liliana Semorile
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Correspondence to Bárbara Mercedes Bravo-Ferrada.

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Bravo-Ferrada, B.M., Hollmann, A., Delfederico, L. et al. Patagonian red wines: selection of Lactobacillus plantarum isolates as potential starter cultures for malolactic fermentation. World J Microbiol Biotechnol 29, 1537–1549 (2013). https://doi.org/10.1007/s11274-013-1337-x

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